1999
In Vitro Selection of Nucleic Acid Enzymes
Breaker R, Kurz M. In Vitro Selection of Nucleic Acid Enzymes. Current Topics In Microbiology And Immunology 1999, 243: 137-158. PMID: 10453642, DOI: 10.1007/978-3-642-60142-2_8.Peer-Reviewed Original ResearchConceptsDiversity of enzymesYears of evolutionNucleic acid enzymesEvolutionary historyNucleic acidsBiochemical functionsDNA substratesMetabolic machineryVitro SelectionProtein enzymesCatalytic functionBiological catalystsAcid enzymesHydrolysis reactionProteinEnzymeNatural functionRibozymeDistinct classesRNAEssential componentReactionMachineryCatalystDiversity
1997
Examination of the catalytic fitness of the hammerhead ribozyme by in vitro selection.
Tang J, Breaker R. Examination of the catalytic fitness of the hammerhead ribozyme by in vitro selection. RNA 1997, 3: 914-25. PMID: 9257650, PMCID: PMC1369536.Peer-Reviewed Original ResearchConceptsConsensus sequenceATP-binding RNA aptamerCatalytic fitnessHammerhead ribozymeAbsence of ATPRNA poolAllosteric ribozymesVitro SelectionRNA aptamersCatalytic functionSequence variantsAllosteric interactionsCombinatorial poolsRibozymeTranscriptionATPRNACatalytic rateSequenceHammerhead domainRibozyme constructsFitnessAllosteric delayPoolSimilar strategies
1996
Are engineered proteins getting competition from RNA?
Breaker R. Are engineered proteins getting competition from RNA? Current Opinion In Biotechnology 1996, 7: 442-448. PMID: 8768905, DOI: 10.1016/s0958-1669(96)80122-4.Peer-Reviewed Original Research
1995
Self-Incorporation of coenzymes by ribozymes
Breaker R, Joyce G. Self-Incorporation of coenzymes by ribozymes. Journal Of Molecular Evolution 1995, 40: 551-558. PMID: 7643406, DOI: 10.1007/bf00160500.Peer-Reviewed Original ResearchConceptsFunctional groupsCoenzyme analoguesAdditional functional groupsChemical functional groupsChemical moietiesTransfer reactionsCovalent attachmentRNA enzymeAbsence of proteinStandard nucleotidesCatalysisCatalytic functionRibozyme activityRNA worldRibozymeMoietyAnaloguesCoenzymeCoA-SHMoleculesReaction
1994
Inventing and improving ribozyme function: Rational design versus iterative selection methods
Breaker R, Joyce G. Inventing and improving ribozyme function: Rational design versus iterative selection methods. Trends In Biotechnology 1994, 12: 268-275. PMID: 7519862, DOI: 10.1016/0167-7799(94)90138-4.Peer-Reviewed Original Research