2002
Role of c-SRC and ERK in acid-induced activation of NHE3
Tsuganezawa H, Sato S, Yamaji Y, Preisig PA, Moe OW, Alpern RJ. Role of c-SRC and ERK in acid-induced activation of NHE3. Kidney International 2002, 62: 41-50. PMID: 12081562, DOI: 10.1046/j.1523-1755.2002.00418.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmonium ChlorideAnimalsCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationJNK Mitogen-Activated Protein KinasesMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesConceptsOKP cellsAcid-induced activationNHE3 activityERK/c-fosC-fos expressionC-Fos activationCytoplasmic pH (pHi) recoveryC-SrcRenal proximal tubulesAcid incubationActivation of NHE3Renal epithelial cellsCultured renal epithelial cellsProximal tubulesChronic acidosisInhibition of MEKNHE3 activationInhibited activationNormal levelsAcid loadImmune complex kinase assayERK kinaseAcidosisPH recoveryEpithelial cells
1997
Intracellular acidosis activates c-Src
Yamaji Y, Tsuganezawa H, Moe OW, Alpern RJ. Intracellular acidosis activates c-Src. American Journal Of Physiology 1997, 272: c886-c893. PMID: 9124524, DOI: 10.1152/ajpcell.1997.272.3.c886.Peer-Reviewed Original ResearchConceptsC-SrcC-Src activationProtein tyrosine phosphatase inhibitorProtein tyrosine kinase pathwaysMedium acidificationTyrosine phosphatase inhibitorProtein tyrosine phosphorylationC-Src activityTyrosine kinase pathwayPhosphatase inhibitorPhosphotyrosine contentCell pH changesTyrosine phosphorylationCytosolic proteinsKinase pathwayRenal proximal tubule cell lineCellular responsesSodium orthovanadateNovel mechanismProximal tubule cell lineCell linesTubule cell lineCell acidificationSignificant activationActivation
1996
Role of tyrosine kinase pathways in ETB receptor activation of NHE3
Chu TS, Tsuganezawa H, Peng Y, Cano A, Yanagisawa M, Alpern RJ. Role of tyrosine kinase pathways in ETB receptor activation of NHE3. American Journal Of Physiology 1996, 271: c763-c771. PMID: 8843705, DOI: 10.1152/ajpcell.1996.271.3.c763.Peer-Reviewed Original ResearchConceptsET-1-induced tyrosine phosphorylationTyrosine kinase pathwayTyrosine phosphorylationKinase pathwayFocal adhesion proteinsIntegral membrane proteinsAntiporter activityClonal cell linesFocal adhesionsMembrane proteinsReceptor activationAdhesion proteinsImmunoprecipitation studiesPhosphorylationCytochalasin DET-1-induced increaseApical membraneDependent pathwayAntiporter activationProteinPaxillinP125FAKCell linesPhosphoproteinPathway
1992
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells.
Horie S, Moe O, Miller RT, Alpern RJ. Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. Journal Of Clinical Investigation 1992, 89: 365-372. PMID: 1310692, PMCID: PMC442861, DOI: 10.1172/jci115594.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityProximal tubule cellsCultured proximal tubule cellsLong-term activationTubule cellsProtein kinase CShort-term activationDose-dependent increaseKinase CActivity 24 hActinomycin DAntiporter activityNa/H antiporterChronic conditionsTwo- to threefold increaseAcute increaseEffect of phorbolProtein kinase C activationChronic effectsTransient increaseMicroM cycloheximidePersistent increaseAlpha-PMAKinase C activationChronic regulator