2018
Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways
Zacchia M, Tian X, Zona E, Alpern RJ, Preisig PA. Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways. Journal Of The American Society Of Nephrology 2018, 29: 1720-1730. PMID: 29678998, PMCID: PMC6054333, DOI: 10.1681/asn.2017121268.Peer-Reviewed Original ResearchMeSH KeywordsAcidsAmmonium ChlorideAnimalsCells, CulturedCitric AcidCSK Tyrosine-Protein KinaseDicarboxylic Acid TransportersEndothelin-1Epithelial CellsFocal Adhesion Kinase 2Kidney Tubules, ProximalMAP Kinase Signaling SystemMiceMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3OpossumsOrganic Anion Transporters, Sodium-DependentPhosphorylationProto-Oncogene Proteins c-rafReceptor, Endothelin BRibosomal Protein S6 Kinases, 90-kDaSrc-Family KinasesTransfectionConceptsET-1/ETEndothelin-1Proximal tubulesCultured opossum kidney cellsAcid stimulationInhibition of Pyk2ET-1 stimulationRenal proximal tubulesNaDC-1Opossum kidney cellsCl loadingC-SrcPhosphorylation of Raf1Urine citratePyk2 knockdownEnd pointAcid loadApical NaERK1/2 pathwayStimulationMiceKidney cellsKidneyPyk2ERK1/2
2010
Acid regulation of NaDC-1 requires a functional endothelin B receptor
Liu L, Zacchia M, Tian X, Wan L, Sakamoto A, Yanagisawa M, Alpern RJ, Preisig PA. Acid regulation of NaDC-1 requires a functional endothelin B receptor. Kidney International 2010, 78: 895-904. PMID: 20703215, DOI: 10.1038/ki.2010.264.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAnimalsBiological TransportCell LineDicarboxylic Acid TransportersDisease Models, AnimalEndothelin B Receptor AntagonistsEndothelin-1Hydrogen-Ion ConcentrationKidneyMiceMice, KnockoutMicrovilliOligopeptidesOpossumsOrganic Anion Transporters, Sodium-DependentPiperidinesProtein Structure, TertiaryReceptor, Endothelin AReceptor, Endothelin BRecombinant Fusion ProteinsSignal TransductionSymportersTime FactorsTransfectionConceptsEndothelin-1Endothelin-B receptor antagonistFunctional endothelin B receptorsStimulatory effectAcid-stimulatory effectET-1 effectsB receptor antagonistNHE3 activityEndothelin B receptorWild-type miceET-1 stimulationMajor physiological stimulusNaDC-1Acid ingestionReceptor antagonistCitrate reabsorptionReceptor expressionCitrate excretionOpossum kidneyAcid loadB receptorAcid feedingPhysiological stimuliStimulationMice
2004
Dietary acid, endothelins, and sleep.
Alpern RJ, Preisig PA. Dietary acid, endothelins, and sleep. Transactions Of The American Clinical And Climatological Association 2004, 115: 385-93; discussion 393-4. PMID: 17060981, PMCID: PMC2263788.Peer-Reviewed Original ResearchConceptsTrafficking of NHE3Proximal tubule cell lineNHE3 activityTubule cell lineSecond intracellular loopEndothelin expressionDietary acidNHE3 abundanceActivation of NHE3OKP cellsProximal tubulesNHE3 mRNAApical membraneHomeostatic responseProtein expressionC-fosReceptor specificityIntracellular loopReceptorsEndothelinCell linesC-JunNHE3C-SrcAcid signaling
2001
Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis
Laghmani K, Preisig P, Moe O, Yanagisawa M, Alpern R. Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis. Journal Of Clinical Investigation 2001, 107: 1563-1569. PMID: 11413164, PMCID: PMC200190, DOI: 10.1172/jci11234.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmoniaAnimalsBicarbonatesChronic DiseaseCitric AcidCulture TechniquesEndothelin-1EndothelinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicProtein PrecursorsReceptor, Endothelin BReceptors, EndothelinRNA, MessengerSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsTg/tgAcid ingestionTitratable acid excretionMetabolic acidosisNHE3 activityAcid excretionReceptor-deficient miceEndothelin B receptorET-1 expressionChronic metabolic acidosisProximal tubule suspensionsAmmonium excretionM endothelinNa/H antiporterC57BL/6 miceApical membrane NHE3Renal epithelial cellsCultured renal epithelial cellsEndothelin-1/Tubule suspensionsMRNA expressionControl dietMiceExcretionEpithelial cells
1997
Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells
Guntupalli J, Onuigbo M, Wall S, Alpern RJ, DuBose TD. Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells. American Journal Of Physiology 1997, 273: c558-c571. PMID: 9277353, DOI: 10.1152/ajpcell.1997.273.2.c558.Peer-Reviewed Original ResearchIntracellular acidosis activates c-Src
Yamaji Y, Tsuganezawa H, Moe OW, Alpern RJ. Intracellular acidosis activates c-Src. American Journal Of Physiology 1997, 272: c886-c893. PMID: 9124524, DOI: 10.1152/ajpcell.1997.272.3.c886.Peer-Reviewed Original ResearchConceptsC-SrcC-Src activationProtein tyrosine phosphatase inhibitorProtein tyrosine kinase pathwaysMedium acidificationTyrosine phosphatase inhibitorProtein tyrosine phosphorylationC-Src activityTyrosine kinase pathwayPhosphatase inhibitorPhosphotyrosine contentCell pH changesTyrosine phosphorylationCytosolic proteinsKinase pathwayRenal proximal tubule cell lineCellular responsesSodium orthovanadateNovel mechanismProximal tubule cell lineCell linesTubule cell lineCell acidificationSignificant activationActivation
1994
Acid activation of immediate early genes in renal epithelial cells.
Yamaji Y, Moe OW, Miller RT, Alpern RJ. Acid activation of immediate early genes in renal epithelial cells. Journal Of Clinical Investigation 1994, 94: 1297-1303. PMID: 8083371, PMCID: PMC295215, DOI: 10.1172/jci117448.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAcidosisAnimalsCell LineCell NucleusDNA-Binding ProteinsEarly Growth Response Protein 1EpitheliumGene Expression RegulationGenes, Immediate-EarlyHydrogen-Ion ConcentrationImmediate-Early ProteinsIonomycinKidney Tubules, ProximalMiceProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junRNA, MessengerTetradecanoylphorbol AcetateTranscription FactorsTranscriptional ActivationConceptsTyrosine kinase pathwayGene activationKinase pathwayMouse proximal tubule cell lineC-JunRate of transcriptionProtein kinase CImmediate early genesRenal epithelial cellsMRNA speciesPretreatment of cellsGene expressionKinase CEarly genesGenesMRNA abundanceProximal tubule cell lineCell linesTubule cell lineRenal tubule cellsEpithelial cellsC-fosGrowth factorCellsMRNA
1992
Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity.
Horie S, Moe O, Yamaji Y, Cano A, Miller RT, Alpern RJ. Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 5236-5240. PMID: 1319056, PMCID: PMC49266, DOI: 10.1073/pnas.89.12.5236.Peer-Reviewed Original ResearchAnimalsCarrier ProteinsCell Line, TransformedChloramphenicol O-AcetyltransferaseGenes, fosGenes, junGlyceraldehyde-3-Phosphate DehydrogenasesHydrogen-Ion ConcentrationKidney Tubules, ProximalKineticsMiceProtein Kinase CProto-Oncogene Proteins c-junRNA, MessengerSimian virus 40Sodium-Hydrogen ExchangersSphingosineTetradecanoylphorbol AcetateTransfection
1991
Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts.
Moe OW, Miller RT, Horie S, Cano A, Preisig PA, Alpern RJ. Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts. Journal Of Clinical Investigation 1991, 88: 1703-1708. PMID: 1658050, PMCID: PMC295708, DOI: 10.1172/jci115487.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityRenal tubule cellsNa/H antiporterMCT cellsRenal epithelial cellsTubule cellsCell linesCultured renal tubule cellsEpithelial cellsMRNA abundanceOpossum kidney cell lineChronic metabolic acidosisAcid preincubationTubule cell lineRat renal cortexH antiporterAntiporter activityUnderlying molecular mechanismsDifferential regulationMetabolic acidosisRenal cortexAcidotic environmentAcid feedingAdaptive increaseKidney cell line