2004
An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3
Licht C, Laghmani K, Yanagisawa M, Preisig PA, Alpern RJ. An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3. Kidney International 2004, 65: 1320-1326. PMID: 15086471, DOI: 10.1111/j.1523-1755.2004.00506.x.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisMetabolic acidosisReverse transcription-polymerase chain reactionProximal tubulesKidney cortexCompetitive reverse transcription-polymerase chain reactionProximal tubule NHE3NHE3 activityPreproET-1 expressionEndothelin B receptorEndothelin-1 expressionET-1 expressionPreproET-1 mRNA abundancePreproET-1 mRNA expressionTranscription-polymerase chain reactionProximal tubule cellsRenal proximal tubulesMRNA abundanceEndothelin-1Control ratsAbdominal aortaAutocrine roleAcidosisTubule cellsAutocrine regulationDietary acid, endothelins, and sleep.
Alpern RJ, Preisig PA. Dietary acid, endothelins, and sleep. Transactions Of The American Clinical And Climatological Association 2004, 115: 385-93; discussion 393-4. PMID: 17060981, PMCID: PMC2263788.Peer-Reviewed Original ResearchConceptsTrafficking of NHE3Proximal tubule cell lineNHE3 activityTubule cell lineSecond intracellular loopEndothelin expressionDietary acidNHE3 abundanceActivation of NHE3OKP cellsProximal tubulesNHE3 mRNAApical membraneHomeostatic responseProtein expressionC-fosReceptor specificityIntracellular loopReceptorsEndothelinCell linesC-JunNHE3C-SrcAcid signaling
2002
Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells
Amemiya M, Kusano E, Muto S, Tabei K, Ando Y, Alpern RJ, Asano Y. Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells. Nephron 2002, 10: 26-33. PMID: 11803202, DOI: 10.1159/000049895.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceOKP cellsNHE3 activityCell pH recoveryRat renal proximal tubulesDibutyryl cAMPThick ascending limbRenal proximal tubulesWestern blot analysisM dibutyryl-cAMPChronic activationPKA-dependent pathwayProximal tubulesAscending limbGlucagonSurface membraneAcid loadNHE3 mRNAProtein abundancePH recoveryExchanger 3Independent mechanismsSignificant increaseBlot analysisP cells
2001
Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis
Laghmani K, Preisig P, Moe O, Yanagisawa M, Alpern R. Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis. Journal Of Clinical Investigation 2001, 107: 1563-1569. PMID: 11413164, PMCID: PMC200190, DOI: 10.1172/jci11234.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmoniaAnimalsBicarbonatesChronic DiseaseCitric AcidCulture TechniquesEndothelin-1EndothelinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicProtein PrecursorsReceptor, Endothelin BReceptors, EndothelinRNA, MessengerSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsTg/tgAcid ingestionTitratable acid excretionMetabolic acidosisNHE3 activityAcid excretionReceptor-deficient miceEndothelin B receptorET-1 expressionChronic metabolic acidosisProximal tubule suspensionsAmmonium excretionM endothelinNa/H antiporterC57BL/6 miceApical membrane NHE3Renal epithelial cellsCultured renal epithelial cellsEndothelin-1/Tubule suspensionsMRNA expressionControl dietMiceExcretionEpithelial cells
2000
Chronic metabolic acidosis increases NaDC-1 mRNA and protein abundance in rat kidney
Aruga S, Wehrli S, Kaissling B, Moe O, Preisig P, Pajor A, Alpern R. Chronic metabolic acidosis increases NaDC-1 mRNA and protein abundance in rat kidney. Kidney International 2000, 58: 206-215. PMID: 10886565, DOI: 10.1046/j.1523-1755.2000.00155.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAcidsAcute DiseaseAlkaliesAmmonium ChlorideAnimalsCarrier ProteinsChronic DiseaseCitratesDicarboxylic Acid TransportersGene ExpressionKidney CortexKidney Tubules, ProximalMaleMembrane ProteinsOrganic Anion Transporters, Sodium-DependentRatsRats, Sprague-DawleyRNA, MessengerSodium BicarbonateSymporters
1999
Incubation of OKP cells in low-K+ media increases NHE3 activity after early decrease in intracellular pH
Amemiya M, Tabei K, Kusano E, Asano Y, Alpern R. Incubation of OKP cells in low-K+ media increases NHE3 activity after early decrease in intracellular pH. American Journal Of Physiology 1999, 276: c711-c716. PMID: 10069999, DOI: 10.1152/ajpcell.1999.276.3.c711.Peer-Reviewed Original ResearchConceptsOKP cellsEarly decreaseNHE3 activityNHE3 protein abundanceTyrosine kinase inhibitorsProximal tubule apical membraneChronic hypokalemiaTyrosine kinase pathwayIntracellular acidosisNHE3 mRNAKinase inhibitorsControl levelsNHE3Herbimycin AKinase pathwayControl mediumPresent studySodium propionateIntracellularCellsAntiporter activityApical membraneHypokalemiaIncubationProtein abundance
1998
Effects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter
Baum M, Dwarakanath V, Alpern R, Moe O. Effects of thyroid hormone on the neonatal renal cortical Na+/H+ antiporter. Kidney International 1998, 53: 1254-1258. PMID: 9573540, PMCID: PMC4131927, DOI: 10.1046/j.1523-1755.1998.00879.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAntithyroid AgentsFemaleHyperthyroidismHypothyroidismKidney CortexKidney Tubules, ProximalMaternal-Fetal ExchangePregnancyPropylthiouracilRatsRats, Sprague-DawleyRNA, MessengerSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Potassium-Exchanging ATPaseTriiodothyronineConceptsThyroid hormonesHyperthyroid neonatesNHE-3 protein abundanceNHE-3 mRNA abundanceDays of lifeLower ratesNeonatal proximal tubuleAntiporter activityHypothyroid neonatesHyperthyroid groupMaturational increaseProximal tubulesPostnatal increaseHyperthyroid ratsBicarbonate absorptionPmol/NeonatesHormonePotential roleBrush borderEuthyroidOne-thirdRatsMRNA abundanceApical membrane
1997
Chronic metabolic acidosis enhances NHE-3 protein abundance and transport activity in the rat thick ascending limb by increasing NHE-3 mRNA.
Laghmani K, Borensztein P, Ambühl P, Froissart M, Bichara M, Moe OW, Alpern RJ, Paillard M. Chronic metabolic acidosis enhances NHE-3 protein abundance and transport activity in the rat thick ascending limb by increasing NHE-3 mRNA. Journal Of Clinical Investigation 1997, 99: 24-30. PMID: 9011571, PMCID: PMC507763, DOI: 10.1172/jci119128.Peer-Reviewed Original Research
1996
Adenosine triphosphate citrate lyase mediates hypocitraturia in rats.
Melnick JZ, Srere PA, Elshourbagy NA, Moe OW, Preisig PA, Alpern RJ. Adenosine triphosphate citrate lyase mediates hypocitraturia in rats. Journal Of Clinical Investigation 1996, 98: 2381-2387. PMID: 8941657, PMCID: PMC507690, DOI: 10.1172/jci119051.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisMetabolic acidosisATP citrate lyase activityATP-citrate lyaseCitrate lyase activityCitrate lyaseHypocitraturiaAcid dietAcidosisCitrate metabolismProtein abundanceRatsMRNA abundanceLyase activityMetabolismPresent studySignificant changesCitrate uptakeInhibitionHypercitraturiaHypokalemiaCytosolic enzymeCompetitive inhibitorActivityImportant roleChronic metabolic acidosis increases NHE3 protein abundance in rat kidney
Ambuhl P, Amemiya M, Danczkay M, Lotscher M, Kaissling B, Moe OW, Preisig PA, Alpern RJ. Chronic metabolic acidosis increases NHE3 protein abundance in rat kidney. American Journal Of Physiology 1996, 271: f917-f925. PMID: 8898023, DOI: 10.1152/ajprenal.1996.271.4.f917.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceChronic metabolic acidosisNHE3 mRNA abundanceMetabolic acidosisThick ascending limbProximal tubulesAscending limbNa/H antiporter activityAcidosis-induced increaseMRNA abundanceNa/H antiporterNHE3 abundanceProtein abundanceAcidosisRat kidneyWestern blotS2 segmentsH antiporterRatsNHE3 isoformPresent studyLimbTubulesGlucocorticoids regulate NHE-3 transcription in OKP cells
Baum M, Amemiya M, Dwarakanath V, Alpern RJ, Moe OW. Glucocorticoids regulate NHE-3 transcription in OKP cells. American Journal Of Physiology 1996, 270: f164-f169. PMID: 8769835, DOI: 10.1152/ajprenal.1996.270.1.f164.Peer-Reviewed Original ResearchConceptsNHE-3 mRNA abundanceGlucocorticoid-mediated increaseOKP cellsDexamethasone-treated cellsNHE-3 mRNAMRNA abundanceTwo- to threefold increaseAntiporter activitySynthetic glucocorticoidProximal tubulesApical proton secretionM dexamethasoneDexamethasoneNHE-3GlucocorticoidsTime courseProton secretionThreefold increasePresent studyCellsGene transcriptionMRNASecretionActivity
1995
Acid incubation increases NHE-3 mRNA abundance in OKP cells
Amemiya M, Yamaji Y, Cano A, Moe OW, Alpern RJ. Acid incubation increases NHE-3 mRNA abundance in OKP cells. American Journal Of Physiology 1995, 269: c126-c133. PMID: 7631739, DOI: 10.1152/ajpcell.1995.269.1.c126.Peer-Reviewed Original ResearchInvolvement of pRB family in TGF beta-dependent epithelial cell hypertrophy.
Franch HA, Shay JW, Alpern RJ, Preisig PA. Involvement of pRB family in TGF beta-dependent epithelial cell hypertrophy. Journal Of Cell Biology 1995, 129: 245-254. PMID: 7698989, PMCID: PMC2120382, DOI: 10.1083/jcb.129.1.245.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Polyomavirus TransformingBlotting, NorthernCell CycleCell DivisionCell LineCells, CulturedEpidermal Growth FactorEpithelial CellsEpitheliumGene ExpressionHumansHypertrophyKidneyKidney TubulesKineticsOncogene Proteins, ViralPapillomavirus E7 ProteinsPhosphorylationProto-Oncogene Proteins c-fosRabbitsRatsRecombinant ProteinsRepressor ProteinsRetinoblastoma ProteinRNA, MessengerTransfectionTransforming Growth Factor betaTumor Suppressor Protein p53ConceptsEpithelial cell hypertrophyNRK-52E cellsCell hypertrophyHPV16 E6TGF betaSV40 large T antigenC-fos mRNA abundanceGrowth factor beta 1SV40 large T antigen expressionLarge T antigenLarge T antigen expressionMechanisms of hypertrophyRenal cell hypertrophyRabbit proximal tubuleActive pRbT-antigen expressionRenal functionT antigenRenal hypertrophyAntigen expressionEpidermal growth factorEGF-induced increaseProximal tubulesHypertrophyHPV16 E7Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat
LaPointe M, Ye M, Moe O, Alpern R, Batlle D. Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat. Kidney International 1995, 47: 78-87. PMID: 7731174, DOI: 10.1038/ki.1995.9.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsCultured aortic vascular smooth muscle cellsWistar/Kyoto ratsAortic vascular smooth muscle cellsWKY ratsHypertensive ratsNominal absenceCultured vascular smooth muscleVascular smooth muscleSmooth muscle cellsCell acidificationRate of intracellularNormotensive controlsKyoto ratsNHE-1 isoformWKY cellsSmooth muscleSHRHigh functional activityRatsMuscle cellsThymidine incorporationPresence of HCO3Exchange activityHCO3-/CO2Molecular cloning of ion transporters: potential clinical implications.
Cano A, Alpern R. Molecular cloning of ion transporters: potential clinical implications. Seminars In Nephrology 1995, 15: 2-8. PMID: 7754253.Peer-Reviewed Original ResearchConceptsIon transportersIntegral membrane proteinsStructure/functionUnderstanding of functionMembrane proteinsMolecular cloningTransporter proteinsHereditary ovalocytosisProteinHyperkalemic periodic paralysisCloningGenetic disordersTransportersMyotonia congenitaMultiple levelsAbnormal structurePotential clinical implicationsPeriodic paralysisEnzymeHereditary spherocytosisRegulationOvalocytosisCystic fibrosisFunction
1994
Acid activation of immediate early genes in renal epithelial cells.
Yamaji Y, Moe OW, Miller RT, Alpern RJ. Acid activation of immediate early genes in renal epithelial cells. Journal Of Clinical Investigation 1994, 94: 1297-1303. PMID: 8083371, PMCID: PMC295215, DOI: 10.1172/jci117448.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAcidosisAnimalsCell LineCell NucleusDNA-Binding ProteinsEarly Growth Response Protein 1EpitheliumGene Expression RegulationGenes, Immediate-EarlyHydrogen-Ion ConcentrationImmediate-Early ProteinsIonomycinKidney Tubules, ProximalMiceProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junRNA, MessengerTetradecanoylphorbol AcetateTranscription FactorsTranscriptional ActivationConceptsTyrosine kinase pathwayGene activationKinase pathwayMouse proximal tubule cell lineC-JunRate of transcriptionProtein kinase CImmediate early genesRenal epithelial cellsMRNA speciesPretreatment of cellsGene expressionKinase CEarly genesGenesMRNA abundanceProximal tubule cell lineCell linesTubule cell lineRenal tubule cellsEpithelial cellsC-fosGrowth factorCellsMRNAEffect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA
Baum M, Moe OW, Gentry DL, Alpern RJ. Effect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA. American Journal Of Physiology 1994, 267: f437-f442. PMID: 8092257, DOI: 10.1152/ajprenal.1994.267.3.f437.Peer-Reviewed Original ResearchConceptsNHE-3 mRNA abundanceProximal tubulesNHE-3NHE-1MRNA abundanceChronic systemic administrationAdministration of dexamethasoneEffects of glucocorticoidsProximal tubule acidificationNHE-1 mRNAUnits/minChronic dexamethasoneTubule acidificationAcute increaseAntiporter activitySystemic administrationGlucocorticoid deficiencyIntravenous administrationBicarbonate absorptionMicrograms/DexamethasoneAntiporter isoformsDexamethasone occursRenal brush-border membrane vesiclesAdministration
1993
Renin expression in renal proximal tubule.
Moe OW, Ujiie K, Star RA, Miller RT, Widell J, Alpern RJ, Henrich WL. Renin expression in renal proximal tubule. Journal Of Clinical Investigation 1993, 91: 774-779. PMID: 7680667, PMCID: PMC288027, DOI: 10.1172/jci116296.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, SouthernCells, CulturedCloning, MolecularColforsinEnalaprilIn Vitro TechniquesIsoproterenolKidney Tubules, ProximalKineticsMaleMolecular Sequence DataMuscle, Smooth, VascularOligodeoxyribonucleotidesPoly APolymerase Chain ReactionRabbitsRatsRats, Sprague-DawleyReninRNARNA, MessengerTranscription, GeneticConceptsProximal tubule cellsCultured proximal tubule cellsRenin-angiotensin systemProximal tubulesRenal proximal tubulesTubule cellsRenin activityLocal renin-angiotensin systemPrimary culturesPresence of reninRabbit proximal tubule cellsAngiotensin-converting enzymeMammalian renal proximal tubulePrevious immunohistochemical studiesSynthesized reninAngiotensin receptorsAngiotensin IIRenin contentPolymerase chain reactionRenin expressionImmunohistochemical studyM isoproterenolM forskolinNormal ratsRenin mRNACell pH and transepithelial H/HCO3 transport in the renal proximal tubule
Krapf R, Alpern R. Cell pH and transepithelial H/HCO3 transport in the renal proximal tubule. The Journal Of Membrane Biology 1993, 131: 1-10. PMID: 8381871, DOI: 10.1007/bf02258529.Peer-Reviewed Original Research
1992
Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity.
Horie S, Moe O, Yamaji Y, Cano A, Miller RT, Alpern RJ. Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 5236-5240. PMID: 1319056, PMCID: PMC49266, DOI: 10.1073/pnas.89.12.5236.Peer-Reviewed Original ResearchAnimalsCarrier ProteinsCell Line, TransformedChloramphenicol O-AcetyltransferaseGenes, fosGenes, junGlyceraldehyde-3-Phosphate DehydrogenasesHydrogen-Ion ConcentrationKidney Tubules, ProximalKineticsMiceProtein Kinase CProto-Oncogene Proteins c-junRNA, MessengerSimian virus 40Sodium-Hydrogen ExchangersSphingosineTetradecanoylphorbol AcetateTransfection