2018
Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways
Zacchia M, Tian X, Zona E, Alpern RJ, Preisig PA. Acid Stimulation of the Citrate Transporter NaDC-1 Requires Pyk2 and ERK1/2 Signaling Pathways. Journal Of The American Society Of Nephrology 2018, 29: 1720-1730. PMID: 29678998, PMCID: PMC6054333, DOI: 10.1681/asn.2017121268.Peer-Reviewed Original ResearchMeSH KeywordsAcidsAmmonium ChlorideAnimalsCells, CulturedCitric AcidCSK Tyrosine-Protein KinaseDicarboxylic Acid TransportersEndothelin-1Epithelial CellsFocal Adhesion Kinase 2Kidney Tubules, ProximalMAP Kinase Signaling SystemMiceMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3OpossumsOrganic Anion Transporters, Sodium-DependentPhosphorylationProto-Oncogene Proteins c-rafReceptor, Endothelin BRibosomal Protein S6 Kinases, 90-kDaSrc-Family KinasesTransfectionConceptsET-1/ETEndothelin-1Proximal tubulesCultured opossum kidney cellsAcid stimulationInhibition of Pyk2ET-1 stimulationRenal proximal tubulesNaDC-1Opossum kidney cellsCl loadingC-SrcPhosphorylation of Raf1Urine citratePyk2 knockdownEnd pointAcid loadApical NaERK1/2 pathwayStimulationMiceKidney cellsKidneyPyk2ERK1/2
2004
An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3
Licht C, Laghmani K, Yanagisawa M, Preisig PA, Alpern RJ. An autocrine role for endothelin-1 in the regulation of proximal tubule NHE3. Kidney International 2004, 65: 1320-1326. PMID: 15086471, DOI: 10.1111/j.1523-1755.2004.00506.x.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisMetabolic acidosisReverse transcription-polymerase chain reactionProximal tubulesKidney cortexCompetitive reverse transcription-polymerase chain reactionProximal tubule NHE3NHE3 activityPreproET-1 expressionEndothelin B receptorEndothelin-1 expressionET-1 expressionPreproET-1 mRNA abundancePreproET-1 mRNA expressionTranscription-polymerase chain reactionProximal tubule cellsRenal proximal tubulesMRNA abundanceEndothelin-1Control ratsAbdominal aortaAutocrine roleAcidosisTubule cellsAutocrine regulationOKP cells express the Na-dicarboxylate cotransporter NaDC-1
Aruga S, Pajor AM, Nakamura K, Liu L, Moe OW, Preisig PA, Alpern RJ. OKP cells express the Na-dicarboxylate cotransporter NaDC-1. American Journal Of Physiology - Cell Physiology 2004, 287: c64-c72. PMID: 14973148, DOI: 10.1152/ajpcell.00061.2003.Peer-Reviewed Original ResearchConceptsCitrate reabsorptionOKP cellsProximal tubulesDependent citrate uptakeOpossum kidney cell lineUrinary citrate concentrationChronic metabolic acidosisRate of reabsorptionRenal proximal tubulesMetabolic acidosisStone formationKidney stonesKidney cell lineCotransporter 1ReabsorptionCell linesCitrate transportMRNA abundanceXenopus oocytesAcid regulationTubulesNaDC-1Citrate uptakeSuccinate transportHypocitraturia
2002
Role of c-SRC and ERK in acid-induced activation of NHE3
Tsuganezawa H, Sato S, Yamaji Y, Preisig PA, Moe OW, Alpern RJ. Role of c-SRC and ERK in acid-induced activation of NHE3. Kidney International 2002, 62: 41-50. PMID: 12081562, DOI: 10.1046/j.1523-1755.2002.00418.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmonium ChlorideAnimalsCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationJNK Mitogen-Activated Protein KinasesMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesConceptsOKP cellsAcid-induced activationNHE3 activityERK/c-fosC-fos expressionC-Fos activationCytoplasmic pH (pHi) recoveryC-SrcRenal proximal tubulesAcid incubationActivation of NHE3Renal epithelial cellsCultured renal epithelial cellsProximal tubulesChronic acidosisInhibition of MEKNHE3 activationInhibited activationNormal levelsAcid loadImmune complex kinase assayERK kinaseAcidosisPH recoveryEpithelial cellsGlucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells
Amemiya M, Kusano E, Muto S, Tabei K, Ando Y, Alpern RJ, Asano Y. Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells. Nephron 2002, 10: 26-33. PMID: 11803202, DOI: 10.1159/000049895.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceOKP cellsNHE3 activityCell pH recoveryRat renal proximal tubulesDibutyryl cAMPThick ascending limbRenal proximal tubulesWestern blot analysisM dibutyryl-cAMPChronic activationPKA-dependent pathwayProximal tubulesAscending limbGlucagonSurface membraneAcid loadNHE3 mRNAProtein abundancePH recoveryExchanger 3Independent mechanismsSignificant increaseBlot analysisP cells
1995
Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb
Amemiya M, Loffing J, Lötscher M, Kaissling B, Alpern R, Moe O. Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb. Kidney International 1995, 48: 1206-1215. PMID: 8569082, DOI: 10.1038/ki.1995.404.Peer-Reviewed Original ResearchConceptsThick ascending limbAscending limbNHE-3H secretionNa/H exchanger isoformsProximal tubulesCortical thick ascending limbNa/H exchange activityS3 proximal tubuleDistal convoluted tubuleRat renal proximal tubulesNa/H exchangeH exchange activityRenal proximal tubulesRat NHE-3Apical membraneAbsent stainingMock-transfected cellsCortical membranesConvoluted tubulesInner stripeStrong stainingWeak stainingOuter medullaNa absorptionRenal Proximal Tubule Response to Acid
Alpern R, Yamaji Y, Amemiya M, Cano A, Preisig P, Miller, Moe O. Renal Proximal Tubule Response to Acid. Physiology 1995, 10: 77-81. DOI: 10.1152/physiologyonline.1995.10.2.77.Peer-Reviewed Original Research
1993
Renin expression in renal proximal tubule.
Moe OW, Ujiie K, Star RA, Miller RT, Widell J, Alpern RJ, Henrich WL. Renin expression in renal proximal tubule. Journal Of Clinical Investigation 1993, 91: 774-779. PMID: 7680667, PMCID: PMC288027, DOI: 10.1172/jci116296.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, SouthernCells, CulturedCloning, MolecularColforsinEnalaprilIn Vitro TechniquesIsoproterenolKidney Tubules, ProximalKineticsMaleMolecular Sequence DataMuscle, Smooth, VascularOligodeoxyribonucleotidesPoly APolymerase Chain ReactionRabbitsRatsRats, Sprague-DawleyReninRNARNA, MessengerTranscription, GeneticConceptsProximal tubule cellsCultured proximal tubule cellsRenin-angiotensin systemProximal tubulesRenal proximal tubulesTubule cellsRenin activityLocal renin-angiotensin systemPrimary culturesPresence of reninRabbit proximal tubule cellsAngiotensin-converting enzymeMammalian renal proximal tubulePrevious immunohistochemical studiesSynthesized reninAngiotensin receptorsAngiotensin IIRenin contentPolymerase chain reactionRenin expressionImmunohistochemical studyM isoproterenolM forskolinNormal ratsRenin mRNA