2024
1734-P: ADP Privation of Human Beta-Cell Mitochondria by Pyruvate Kinase
RUZ-MALDONADO I, CARDONE R, KIBBEY R. 1734-P: ADP Privation of Human Beta-Cell Mitochondria by Pyruvate Kinase. Diabetes 2024, 73 DOI: 10.2337/db24-1734-p.Peer-Reviewed Original ResearchPyruvate kinaseBeta-cell mitochondriaInhibition of OxPhosBeta-cellsHuman beta-cellsModulation of enzymesIntramitochondrial ADPPhosphoenolpyruvate inhibitionADP availabilityADP/ATP exchangeKATP channelsPhosphoenolpyruvate metabolismOxidative phosphorylationPhosphoenolpyruvateMitochondrial functionOXPHOSSubstrate transportComplex IATP/ADP ratioHuman isletsMitochondrial couplingOxygen consumption ratePyruvateMitochondriaDose-dependent manner
2020
Pyruvate Kinase Controls Signal Strength in the Insulin Secretory Pathway
Lewandowski SL, Cardone RL, Foster HR, Ho T, Potapenko E, Poudel C, VanDeusen HR, Sdao SM, Alves TC, Zhao X, Capozzi ME, de Souza AH, Jahan I, Thomas CJ, Nunemaker CS, Davis DB, Campbell JE, Kibbey RG, Merrins MJ. Pyruvate Kinase Controls Signal Strength in the Insulin Secretory Pathway. Cell Metabolism 2020, 32: 736-750.e5. PMID: 33147484, PMCID: PMC7685238, DOI: 10.1016/j.cmet.2020.10.007.Peer-Reviewed Original ResearchConceptsPyruvate kinaseATP/ADPΒ-cell metabolismAppropriate insulin secretionPotential therapeutic routeSecretory pathwayMitochondrial fuelsPancreatic β-cellsInsulin secretory pathwayOxidative phosphorylationCell metabolismNutrient metabolismPhosphoenolpyruvateCell sensingPK activatorΒ-cellsCell functionInsulin secretionPK activityOxidative functionMembrane depolarizationMitochondriaPK activationΒ-cell functionADP