1998
Local Regulation of Macrophage Subsets in the Adult Rat Testis: Examination of the Roles of the Seminiferous Tubules, Testosterone, and Macrophage-Migration Inhibitory Factor1
Meinhardt A, Bacher M, Metz C, Bucala R, Wreford N, Lan H, Atkins R, Hedger M. Local Regulation of Macrophage Subsets in the Adult Rat Testis: Examination of the Roles of the Seminiferous Tubules, Testosterone, and Macrophage-Migration Inhibitory Factor1. Biology Of Reproduction 1998, 59: 371-378. PMID: 9687310, DOI: 10.1095/biolreprod59.2.371.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorSeminiferous tubule functionLeydig cell functionAdult rat testisMacrophage subsetsMacrophage numbersT implantsTubule functionAbdominal testesRat testisLeydig cellsResident macrophagesCytokine macrophage migration inhibitory factorMonocyte/macrophage subsetsCell functionMacrophage surface antigensResident macrophage numbersMacrophage migration inhibitoryMonoclonal antibody ED2MIF levelsLack of expressionSurface antigenInhibitory factorTestosterone implantsScrotal testesInvolvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis
Leech M, Metz C, Santos L, Peng T, Holdsworth S, Bucala R, Morand E. Involvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis. Arthritis & Rheumatism 1998, 41: 910-917. PMID: 9588744, DOI: 10.1002/1529-0131(199805)41:5<910::aid-art19>3.0.co;2-e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArthritis, ExperimentalBlotting, WesternCell CountCells, CulturedDose-Response Relationship, DrugEdemaEnzyme-Linked Immunosorbent AssayImmunohistochemistryMacrophage Migration-Inhibitory FactorsMacrophagesMaleNeutralization TestsRatsRats, Sprague-DawleySynovial MembraneConceptsMacrophage migration inhibitory factorRat adjuvant arthritisAdjuvant arthritisMigration inhibitory factorSynovial macrophagesRat synoviumLevels of MIFRole of MIFInhibitory factorMonoclonal antibodiesArthritis clinical scoresT cell accumulationDose-dependent inhibitionEnzyme-linked immunosorbentMIF treatmentAdjuvant injectionMAb treatmentClinical scoresED-1Leukocyte numbersSpecific monoclonal antibodiesClinical aspectsT cellsArthritisMacrophage activationContribution of advanced glycosylation to the amyloidogenicity of islet amyloid polypeptide
Kapurniotu A, Bernhagen J, Greenfield N, Al‐Abed Y, Teichberg S, Frank R, Voelter W, Bucala R. Contribution of advanced glycosylation to the amyloidogenicity of islet amyloid polypeptide. The FEBS Journal 1998, 251: 208-216. PMID: 9492286, DOI: 10.1046/j.1432-1327.1998.2510208.x.Peer-Reviewed Original ResearchConceptsAmyloid formationIslet amyloid polypeptidePre-formed aggregatesAdvanced glycosylation end productsIAPP amyloid formationAlzheimer's disease amyloid peptideCircular dichroism spectropolarimetryNucleation-dependent polymerization mechanismBeta-sheet conformationIAPP aggregationBiophysical techniquesIAPP aggregatesHigh molecular massFormation of amyloidAmyloid structuresGreen-gold birefringenceProtein amino groupsCharacteristic fibrillar structureIslet amyloid formationSeedsFibrillar amyloid structuresGlycosylationPolymerization mechanismNon-enzymatic additionPeptide aggregation
1995
MIF as a glucocorticoid-induced modulator of cytokine production
Calandra T, Bernhagen J, Metz C, Spiegel L, Bacher M, Donnelly T, Cerami A, Bucala R. MIF as a glucocorticoid-induced modulator of cytokine production. Nature 1995, 377: 68-71. PMID: 7659164, DOI: 10.1038/377068a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell LineCytokinesDexamethasoneEnzyme-Linked Immunosorbent AssayFemaleGlucocorticoidsHumansHydrocortisoneImmunityInflammationLipopolysaccharidesMacrophage ActivationMacrophage Migration-Inhibitory FactorsMacrophagesMiceMice, Inbred BALB CRatsRats, Sprague-DawleyRecombinant ProteinsShock, SepticT-LymphocytesConceptsMacrophage migration inhibitory factorCounter-regulatory systemsMigration inhibitory factorProtein macrophage migration inhibitory factorGlucocorticoid protectionLethal endotoxaemiaMIF productionCytokine productionCytokine secretionMacrophage cytokinesEndogenous mediatorsImmunosuppressive propertiesImmune responseGlucocorticoid hormonesInhibitory factorHormonal systemsCritical mediatorUnexpected findingMediatorsVital functionsEndotoxaemiaCytokinesGlucocorticoidsMonocytesPituitary