2022
CD74 as a regulator of transcription in normal B cells
David K, Friedlander G, Pellegrino B, Radomir L, Lewinsky H, Leng L, Bucala R, Becker-Herman S, Shachar I. CD74 as a regulator of transcription in normal B cells. Cell Reports 2022, 41: 111572. PMID: 36323260, DOI: 10.1016/j.celrep.2022.111572.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorNormal B cellsB cellsCytokine macrophage migration inhibitory factorRegulators of transcriptionChronic lymphocytic leukemia cellsMigration inhibitory factorNovel therapeutic pathwaysInhibition of transcriptionLymphocytic leukemia cellsTumor suppressor geneTranscriptional regulatorsTranscription factorsTherapeutic pathwaysCLL cellsFuture treatmentIntracellular domainOncogenic transformationMalignant cellsInhibitory factorRegulatory functionsPromoter areaLeukemia cellsTranscriptionGenes
2021
MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model
Tilstam PV, Schulte W, Holowka T, Kim BS, Nouws J, Sauler M, Piecychna M, Pantouris G, Lolis E, Leng L, Bernhagen J, Fingerle-Rowson G, Bucala R. MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model. Journal Of Clinical Investigation 2021, 131: e127171. PMID: 34850744, PMCID: PMC8631602, DOI: 10.1172/jci127171.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCytokinesDisease Models, AnimalFemaleFlow CytometryGene Expression ProfilingInflammationIntramolecular OxidoreductasesLeukocyte CountMacrophage Migration-Inhibitory FactorsMacrophagesMacrophages, PeritonealMaleMiceMice, Inbred C57BLMice, TransgenicPeritoneal LavagePhenotypeProtein BindingRNA-SeqSepsisSignal TransductionConceptsMacrophage migration inhibitory factorSmall peritoneal macrophagesLarge peritoneal macrophagesPolymicrobial sepsisPeritoneal macrophagesMIF receptor CD74MIF promoter polymorphismsMIF-2Migration inhibitory factorPolymicrobial sepsis modelMIF deficiencyAdoptive transferSeptic shockSurvival benefitInfectious insultsMIF antibodyExcessive inflammationInflammatory cytokinesReceptor CD74Sepsis modelProtective effectPeritoneal cavityDifferent infectionsPromoter polymorphismInflammatory macrophages
2000
A most interesting factor
Bucala R. A most interesting factor. Nature 2000, 408: 146-147. PMID: 11089953, DOI: 10.1038/35041654.Peer-Reviewed Original Research
1996
NMR characterization of structure, backbone dynamics, and glutathione binding of the human macrophage migration inhibitory factor (MIF)
Mühlhahn P, Czisch M, Georgescu J, Renner C, Ross A, Holak T, Bernhagen J, Bucala R. NMR characterization of structure, backbone dynamics, and glutathione binding of the human macrophage migration inhibitory factor (MIF). Protein Science 1996, 5: 2095-2103. PMID: 8897610, PMCID: PMC2143260, DOI: 10.1002/pro.5560051016.Peer-Reviewed Original Research
1984
The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo
BUCALA R, FISHMAN J, CERAMI A. The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo. The FEBS Journal 1984, 140: 593-598. PMID: 6723653, DOI: 10.1111/j.1432-1033.1984.tb08143.x.Peer-Reviewed Original ResearchConceptsMembrane proteinsCellular proteinsCell lifeRed cell lifeLysine residuesProteinCovalent adductsHydrophobic chromatographyAcid-precipitable radioactivityRate of incorporationStable covalent adductCellsReverse-phase high-pressure liquid chromatographyElevated levelsResiduesAdduct formationRed cellsElectrophoresisVitro