2021
Curcumin ameliorates IL‐1β‐induced apoptosis by activating autophagy and inhibiting the NF‐κB signaling pathway in rat primary articular chondrocytes
Chen T, Zhou R, Chen Y, Fu W, Wei X, Ma G, Hu W, Lu C. Curcumin ameliorates IL‐1β‐induced apoptosis by activating autophagy and inhibiting the NF‐κB signaling pathway in rat primary articular chondrocytes. Cell Biology International 2021, 45: 976-988. PMID: 33377585, DOI: 10.1002/cbin.11541.Peer-Reviewed Original ResearchConceptsIL-1βChondrocyte apoptosisProtective effectNF-κBPrimary articular chondrocytesNF-κB inhibitor pyrrolidine dithiocarbamatePhosphorylation levelsArticular chondrocytesCurcumin-mediated protectionWnt/β-catenin inhibitorInhibitor pyrrolidine dithiocarbamateIL-1β stimulationArticular chondrocyte apoptosisArticular cartilage damageΒ-catenin inhibitorMechanism of curcuminArticular cartilage diseaseSynthase kinase-3βCurcumin pretreatmentRheumatoid arthritisΚB p65Chondrocyte injuryInterleukin-1βCartilage damageXAV-939
2019
Effects of autophagy on apoptosis of articular chondrocytes in adjuvant arthritis rats
Zhou R, Zhu F, Wu X, Song S, Chen Y, Zhu C, Dai B, Qian X, Wang K, Hu W, Chen F. Effects of autophagy on apoptosis of articular chondrocytes in adjuvant arthritis rats. Journal Of Cellular And Molecular Medicine 2019, 23: 7879-7884. PMID: 31508906, PMCID: PMC6815768, DOI: 10.1111/jcmm.14629.Peer-Reviewed Original ResearchConceptsRheumatoid arthritisEffect of autophagyChondrocyte apoptosisAdjuvant arthritisDevelopment of RATreatment of RASystemic autoimmune diseaseAdjuvant arthritis ratsArticular chondrocyte apoptosisAutophagy activator rapamycinArticular cartilage injuryArticular chondrocytesRat articular cartilageArthritis ratsJoint deformityAutoimmune diseasesAA ratsCartilage injuryJoint functionChondrocyte autophagyAutophagy inhibitorPotential targetArthritisApoptosisRats
2017
Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes
Xie Y, Li Y, Zhou R, Dai B, Qian Y, Wu X, Ge J, Hu W, Chen F. Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes. Molecular And Cellular Biochemistry 2017, 443: 181-191. PMID: 29273849, DOI: 10.1007/s11010-017-3223-6.Peer-Reviewed Original ResearchConceptsRat articular chondrocytesRheumatoid arthritisEffect of autophagyPathogenesis of RAArticular chondrocytesProgression of RAInhibition of ASIC1aAcid-sensing ion channel 1aMultiple pathogenic factorsDegenerative joint diseaseArticular chondrocyte apoptosisRat knee jointsIon channel 1aShort hairpin RNAPrecise etiologyJoint diseasePathogenic factorsKnee jointPsalmotoxin-1Chondrocyte apoptosisPrimary articular chondrocytesChannel 1aASIC1aAutophagy fluxBeclin-1ASIC2a overexpression enhances the protective effect of PcTx1 and APETx2 against acidosis-induced articular chondrocyte apoptosis and cytotoxicity
Zhou R, Ni W, Dai B, Wu X, Wang Z, Xie Y, Wang Z, Yang W, Ge J, Hu W, Chen F. ASIC2a overexpression enhances the protective effect of PcTx1 and APETx2 against acidosis-induced articular chondrocyte apoptosis and cytotoxicity. Gene 2017, 642: 230-240. PMID: 29141196, DOI: 10.1016/j.gene.2017.11.034.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion Channel BlockersAcid Sensing Ion ChannelsAcidosisAlkanesulfonic AcidsAnimalsApoptosisCells, CulturedChondrocytesCnidarian VenomsCollagen Type IIDrug SynergismGene Expression RegulationGenetic VectorsMAP Kinase Signaling SystemMorpholinesPeptidesPlasmidsRatsSpider VenomsConceptsAcid-sensing ion channel 1aArticular chondrocyte apoptosisChondrocyte apoptosisChondrocyte cytotoxicityRheumatoid arthritisProtective effectExpression of ASIC1aAcidosis-induced injuryERK1/2 MAPK signaling pathwaysIon channel 1aAlone groupNeuroprotective effectsChondroprotective effectsMAPK signaling pathwaysProtective roleType II collagenChannel 1aNeuronal cellsAPETx2ASIC2aPcTx1Phosphorylated ERK1/2Pathological charactersInduced apoptosisII collagenInterleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel
Zhou R, Dai B, Xie Y, Wu X, Wang Z, Li Y, Wang Z, Zu S, Ge J, Chen F. Interleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel. Biochimica Et Biophysica Acta (BBA) - Molecular Basis Of Disease 2017, 1864: 162-177. PMID: 28986307, DOI: 10.1016/j.bbadis.2017.10.004.Peer-Reviewed Original ResearchConceptsAcid-sensing ion channel 1aIL-1βRheumatoid arthritisInterleukin-1βNF-κB inhibitor ammonium pyrrolidinedithiocarbamateNF-κB DNA-binding activityPrimary articular chondrocytesProinflammatory cytokine interleukin-1βAcidosis-induced cytotoxicityCytokines interleukin-1βTumor necrosis factorImportant pathological characteristicNF-κB pathwayArticular chondrocyte apoptosisCaspase-3/9 expressionIon channel 1aNuclear factor-kappaBPleiotropic biological effectsArticular chondrocytesAgent BAPTA-AMASIC1a expressionChondrocyte cytotoxicityΚB expressionAdjuvant arthritisPathological characteristics
2015
Interleukin-6 enhances acid-induced apoptosis via upregulating acid-sensing ion channel 1a expression and function in rat articular chondrocytes
Zhou R, Wu X, Wang Z, Ge J, Chen F. Interleukin-6 enhances acid-induced apoptosis via upregulating acid-sensing ion channel 1a expression and function in rat articular chondrocytes. International Immunopharmacology 2015, 29: 748-760. PMID: 26359543, DOI: 10.1016/j.intimp.2015.08.044.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion ChannelsAcidsAnimalsAntibodies, Monoclonal, HumanizedApoptosisApoptosis Regulatory ProteinsArthritis, ExperimentalCartilage, ArticularChondrocytesDose-Response Relationship, DrugEnzyme ActivationInterleukin-6L-Lactate DehydrogenaseMalePeptidesRatsRats, Sprague-DawleySpider VenomsUp-RegulationConceptsInflammatory cytokine interleukin-6Acid-sensing ion channelsArticular chondrocyte apoptosisRat articular chondrocytesChondrocyte apoptosisRheumatoid arthritisAcid-induced cell injuryArticular chondrocytesAdjuvant arthritis ratsPrimary rat articular chondrocytesChronic inflammatory diseaseCytokine interleukin-6IL-6 receptorJAK2/STAT3Dose-dependent mannerArthritis ratsInflammatory diseasesInterleukin-6Specific antagonistCell injuryNF-κBAcid-induced apoptosisLDH releaseArthritic cartilageDownregulated expression