2017
Chapter 8 Characterization of GPCR signaling in hypoxia
Chakraborty R, Sikarwar A, Hinton M, Dakshinamurti S, Chelikani P. Chapter 8 Characterization of GPCR signaling in hypoxia. Methods In Cell Biology 2017, 142: 101-110. PMID: 28964329, DOI: 10.1016/bs.mcb.2017.07.005.Peer-Reviewed Original ResearchConceptsThromboxane A2 receptorG protein-coupled receptorsPrimary pulmonary artery smooth muscle cellsPulmonary artery smooth muscle cellsArtery smooth muscle cellsPersistent pulmonary hypertensionPulmonary arterial responsesKey G‐protein‐coupled receptorsSmooth muscle cellsPulmonary hypertensionProtein-coupled receptorsArterial responseTissue hypoxiaCardiovascular diseaseA2 receptorsT cellsProstanoid receptorsCell responsesMuscle cellsHypoxic experimentsGPCR signalingHypoxiaHEK293T cellsReceptorsOxygen supply
2016
The Pharmacochaperone Activity of Quinine on Bitter Taste Receptors
Upadhyaya J, Chakraborty R, Shaik F, Jaggupilli A, Bhullar R, Chelikani P. The Pharmacochaperone Activity of Quinine on Bitter Taste Receptors. PLOS ONE 2016, 11: e0156347. PMID: 27223611, PMCID: PMC4880206, DOI: 10.1371/journal.pone.0156347.Peer-Reviewed Original ResearchConceptsBitter taste receptorsBitter taste signal transductionTaste receptorsTaste signal transductionControl untreated cellsQuinine treatmentAgonist treatmentCell surface expressionCalcium responsePharmacological techniquesAgonist activityTaste sensationBitter agonistsBasic taste sensationsReceptor internalizationSurface expressionUntreated cellsQuinineTreatmentDesensitizationReceptorsSignal transductionObserved actions
2013
New Insights into Structural Determinants for Prostanoid Thromboxane A2 Receptor- and Prostacyclin Receptor-G Protein Coupling
Chakraborty R, Pydi SP, Gleim S, Bhullar RP, Hwa J, Dakshinamurti S, Chelikani P. New Insights into Structural Determinants for Prostanoid Thromboxane A2 Receptor- and Prostacyclin Receptor-G Protein Coupling. Molecular And Cellular Biology 2013, 33: 184-193. PMID: 23109431, PMCID: PMC3554117, DOI: 10.1128/mcb.00725-12.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesCalciumFluorescent Antibody TechniqueGTP-Binding ProteinsHEK293 CellsHumansInositol 1,4,5-TrisphosphateLigandsModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedNonlinear DynamicsProtein BindingProtein ConformationReceptors, EpoprostenolReceptors, Thromboxane A2, Prostaglandin H2Signal TransductionConceptsG protein-coupled receptorsG protein specificityThromboxane A2 receptorProtein interactionsProtein specificityIntracellular loopChimeric receptorsG proteinsGPCR-G protein interactionsProtein couplingHeterotrimeric G proteinsCognate G-proteinSite-directed mutagenesisNew molecular insightsProtein-coupled receptorsG protein couplingReceptor-G protein interactionG protein activationA2 receptorsReceptor-G protein couplingStructural basisMolecular insightsProtein activationMolecular natureStructural determinants
2012
Site-Directed Mutations and the Polymorphic Variant Ala160Thr in the Human Thromboxane Receptor Uncover a Structural Role for Transmembrane Helix 4
Chakraborty R, Pydi SP, Gleim S, Dakshinamurti S, Hwa J, Chelikani P. Site-Directed Mutations and the Polymorphic Variant Ala160Thr in the Human Thromboxane Receptor Uncover a Structural Role for Transmembrane Helix 4. PLOS ONE 2012, 7: e29996. PMID: 22272267, PMCID: PMC3260207, DOI: 10.1371/journal.pone.0029996.Peer-Reviewed Original ResearchMeSH Keywords15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic AcidAlanineAmino Acid SequenceAnimalsBinding SitesBinding, CompetitiveBridged Bicyclo Compounds, HeterocyclicCalciumChlorocebus aethiopsCOS CellsFatty Acids, UnsaturatedGlycineHEK293 CellsHumansHydrazinesMicroscopy, FluorescenceModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMutant ProteinsMutation, MissensePolymorphism, Single NucleotideProtein Structure, SecondaryRadioligand AssayReceptors, Thromboxane A2, Prostaglandin H2Structure-Activity RelationshipTemperature