2016
The Pharmacochaperone Activity of Quinine on Bitter Taste Receptors
Upadhyaya J, Chakraborty R, Shaik F, Jaggupilli A, Bhullar R, Chelikani P. The Pharmacochaperone Activity of Quinine on Bitter Taste Receptors. PLOS ONE 2016, 11: e0156347. PMID: 27223611, PMCID: PMC4880206, DOI: 10.1371/journal.pone.0156347.Peer-Reviewed Original ResearchConceptsBitter taste receptorsBitter taste signal transductionTaste receptorsTaste signal transductionControl untreated cellsQuinine treatmentAgonist treatmentCell surface expressionCalcium responsePharmacological techniquesAgonist activityTaste sensationBitter agonistsBasic taste sensationsReceptor internalizationSurface expressionUntreated cellsQuinineTreatmentDesensitizationReceptorsSignal transductionObserved actions
2014
Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells
Singh N, Chakraborty R, Bhullar R, Chelikani P. Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells. Biochemical And Biophysical Research Communications 2014, 446: 499-503. PMID: 24613843, DOI: 10.1016/j.bbrc.2014.02.140.Peer-Reviewed Original ResearchConceptsBreast cancer cellsCancer cellsBitter taste receptorsMammary epithelial cellsMetastatic breast cancer cell line MDA-MB-231Epithelial cellsBreast cancer cell line MDA-MB-231Intracellular calcium mobilizationCancer cell line MDA-MB-231Cell line MDA-MB-231Taste receptorsBitter melon extractNon-cancerous breastCell line MCF-7G protein-coupled receptorsMDA-MB-231Protein-coupled receptorsHuman bitter taste receptorsEstrogen receptorCalcium mobilizationTumor growthMelon extractFlow cytometryMCF-10A.Receptors
2012
Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras
Pydi S, Chakraborty R, Bhullar R, Chelikani P. Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras. Biochemical And Biophysical Research Communications 2012, 430: 179-182. PMID: 23159609, DOI: 10.1016/j.bbrc.2012.11.029.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsBitter taste receptorsTaste receptorsProtein-coupled receptorsBitter taste sensationReceptor expressionLow-level expressionCalcium imagingReceptor functionFlow cytometryReceptorsTaste sensationAllosteric ligand bindingT2RsReceptor chimerasAmino acidsExpressionReceptor structureN-terminal 33N-terminal 33 amino acidsN-terminal sequenceCytometry