Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras
Pydi S, Chakraborty R, Bhullar R, Chelikani P. Role of rhodopsin N-terminus in structure and function of rhodopsin-bitter taste receptor chimeras. Biochemical And Biophysical Research Communications 2012, 430: 179-182. PMID: 23159609, DOI: 10.1016/j.bbrc.2012.11.029.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsBitter taste receptorsTaste receptorsProtein-coupled receptorsBitter taste sensationReceptor expressionLow-level expressionCalcium imagingReceptor functionFlow cytometryReceptorsTaste sensationAllosteric ligand bindingT2RsReceptor chimerasAmino acidsExpressionReceptor structureN-terminal 33N-terminal 33 amino acidsN-terminal sequenceCytometrySite-Directed Mutations and the Polymorphic Variant Ala160Thr in the Human Thromboxane Receptor Uncover a Structural Role for Transmembrane Helix 4
Chakraborty R, Pydi SP, Gleim S, Dakshinamurti S, Hwa J, Chelikani P. Site-Directed Mutations and the Polymorphic Variant Ala160Thr in the Human Thromboxane Receptor Uncover a Structural Role for Transmembrane Helix 4. PLOS ONE 2012, 7: e29996. PMID: 22272267, PMCID: PMC3260207, DOI: 10.1371/journal.pone.0029996.Peer-Reviewed Original ResearchMeSH Keywords15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic AcidAlanineAmino Acid SequenceAnimalsBinding SitesBinding, CompetitiveBridged Bicyclo Compounds, HeterocyclicCalciumChlorocebus aethiopsCOS CellsFatty Acids, UnsaturatedGlycineHEK293 CellsHumansHydrazinesMicroscopy, FluorescenceModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedMutant ProteinsMutation, MissensePolymorphism, Single NucleotideProtein Structure, SecondaryRadioligand AssayReceptors, Thromboxane A2, Prostaglandin H2Structure-Activity RelationshipTemperature