Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte D, Li C, Thomson R, Nix S, Zanjani R, Karp S, Aronson P, Igarashi P. Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity. American Journal Of Physiology 1999, 277: f587-f598. PMID: 10516284, DOI: 10.1152/ajprenal.1999.277.4.f587.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 3Activator protein-2Renal epithelial cellsCAAT boxNuclear proteinsFlanking regionsPromoter activityTissue-specific gene expressionEpithelial cellsElectrophoretic mobility shift assaysKsp-cadherin gene promoterCCAAT/enhancer-binding proteinTranscription initiation siteKidney-specific memberMobility shift assaysConsensus promoter elementsEnhancer-binding proteinCell-specific activityPotential binding sitesLambda bacteriophage clonesLuciferase reporter plasmidHelix proteinsKidney epithelial cellsGATA factorsGC boxKsp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice
Igarashi P, Shashikant C, Thomson R, Whyte D, Liu-Chen S, Ruddle F, Aronson P. Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice. American Journal Of Physiology 1999, 277: f599-f610. PMID: 10516285, DOI: 10.1152/ajprenal.1999.277.4.f599.Peer-Reviewed Original ResearchConceptsKsp-cadherinEscherichia coli lacZ reporter geneTissue-specific memberKsp-cadherin gene promoterRepeat-induced geneTranscriptional start siteTissue-specific expressionDifferentiation-specific expressionBeta-galactosidase enzyme activityTransgene integration sitesKidney-specific expressionLacZ reporter geneTransgenic miceEpithelial cellsKidney-specific activityStart siteRegulatory elementsGene promoterReporter genePronuclear microinjectionKb upstreamUreteric budTubular epithelial cellsDevelopmental expressionIntegration sites