2008
Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI–albumin conjugates
Wisnewski AV, Liu Q, Liu J, Redlich CA. Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI–albumin conjugates. Clinical & Experimental Allergy 2008, 38: 957-967. PMID: 18498542, PMCID: PMC3131007, DOI: 10.1111/j.1365-2222.2008.02982.x.Peer-Reviewed Original ResearchMeSH KeywordsAdipokinesAdministration, InhalationAlbuminsAntigens, CDBlotting, WesternCells, CulturedChemokine CCL2Chitinase-3-Like Protein 1ChitinasesCyanatesEnzyme-Linked Immunosorbent AssayGene ExpressionGlycoproteinsHumansImmunity, InnateIntramolecular OxidoreductasesIsocyanatesLectinsLeukocytes, MononuclearLysosomesMacrophage Migration-Inhibitory FactorsMicroscopy, FluorescenceOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionPolymorphism, GeneticProteinsRNAConceptsPeripheral blood mononuclear cellsInnate immune responseImmune responsePercentage of PBMCHuman peripheral blood mononuclear cellsFlow cytometrySystemic innate immune responseExposure-dependent decreaseMonocyte/macrophage traffickingHuman innate immune responseProtein expressionSpecific inhalation challengeBlood mononuclear cellsAdaptive immune responsesLow-density lipoproteinPattern recognition receptorsInhalation challengeOccupational asthmaClinical spectrumControl antigenMononuclear cellsHDI exposureSerum concentrationsAdaptive immunityGene/protein expression
2004
Isocyanate vapor-induced antigenicity of human albumin
Wisnewski AV, Stowe MH, Cartier A, Liu Q, Liu J, Chen L, Redlich CA. Isocyanate vapor-induced antigenicity of human albumin. Journal Of Allergy And Clinical Immunology 2004, 113: 1178-1184. PMID: 15208602, DOI: 10.1016/j.jaci.2004.03.009.Peer-Reviewed Original ResearchConceptsHDI exposureHuman albuminAuto body shop workersHDI vaporHuman immune systemOccupational asthmaHumoral armSpecific IgEIgG titersHealthy workersImmune responseSerology studiesHuman airwaysImmune systemSignificant associationAntigenic formsShop workersAlbumin conjugatesAsthmaticsAsthmaIgEAntigenicityParallel studyAlbuminSignificant changes
2003
Human γ/δ T-cell proliferation and IFN-γ production induced by hexamethylene diisocyanate
Wisnewski AV, Herrick CA, Liu Q, Chen L, Bottomly K, Redlich CA. Human γ/δ T-cell proliferation and IFN-γ production induced by hexamethylene diisocyanate. Journal Of Allergy And Clinical Immunology 2003, 112: 538-546. PMID: 13679813, DOI: 10.1016/s0091-6749(03)01865-7.Peer-Reviewed Original ResearchConceptsGamma/delta T cellsDelta T cellsHuman immune responseT cellsT cell receptorImmune responseAlpha/beta T cell receptorBeta T cell receptorHuman airway epithelial cellsIFN-γ productionGamma/deltaT cell proliferationAirway epithelial cellsHuman T cell lineT cell linesHuman T cellsCell typesOccupational asthmaCytokine productionPeripheral bloodIL-13IL-5Antigen-mediated selectionAntigen leadIFN-gammaRecent developments in diisocyanate asthma
Liu Q, Wisnewski AV. Recent developments in diisocyanate asthma. Annals Of Allergy Asthma & Immunology 2003, 90: 35-41. PMID: 12772950, DOI: 10.1016/s1081-1206(10)61647-x.Peer-Reviewed Original ResearchConceptsDiisocyanate asthmaPotential long-term adverse effectsMajor histocompatibility complex class II allelesLong-term adverse effectsGlutathione S-transferase polymorphismsImmune-dependent mechanismsAntigen-independent mechanismsLong-term followGenetic factorsHuman immune responseClass II allelesEffective diagnostic testAllergic asthmaAsthma patientsAtopic asthmaDiisocyanate exposureSymptomatic patientsImmune basisSensitized patientsAsthma pathogenesisPrognostic valueImmunologic differencesAirway epitheliumImmune responseAsthma