Distinct polyfunctional CD4+ T cell responses to BCG, ESAT-6 and CFP-10 in tuberculous pleurisy
Li L, Qiao D, Li Q, Zhang X, Lao S, Wu C. Distinct polyfunctional CD4+ T cell responses to BCG, ESAT-6 and CFP-10 in tuberculous pleurisy. Tuberculosis 2011, 92: 63-71. PMID: 22154006, DOI: 10.1016/j.tube.2011.11.004.Peer-Reviewed Original ResearchConceptsPleural fluid cellsESAT-6T cellsMedian fluorescence intensityTuberculous pleurisyCFP-10IL-2Effector/effector memory phenotypeAntigen-specific T cellsFrequent extrapulmonary manifestationMtb-specific antigensEffector memory phenotypeT cell responsesCFP-10 peptidesExtrapulmonary manifestationsPolyfunctional CD4Th1 cytokinesTuberculosis infectionMemory phenotypeInflammatory cellsTh1 cellsSpecific antigenLocal controlCell responsesFollowing StimulationB lymphocytes that migrate to tuberculous pleural fluid via the SDF‐1/CXCR4 axis actively respond to antigens specific for Mycobacterium tuberculosis
Feng L, Li L, Liu Y, Qiao D, Li Q, Fu X, Wang H, Lao S, Wu C. B lymphocytes that migrate to tuberculous pleural fluid via the SDF‐1/CXCR4 axis actively respond to antigens specific for Mycobacterium tuberculosis. European Journal Of Immunology 2011, 41: 3261-3269. PMID: 21818756, DOI: 10.1002/eji.201141625.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAntigens, BacterialB-Lymphocyte SubsetsB-LymphocytesCell SeparationChemokine CXCL12Chemotaxis, LeukocyteEnzyme-Linked Immunosorbent AssayFemaleFlow CytometryHumansLymphocyte ActivationMaleMiddle AgedMycobacterium tuberculosisReceptors, CXCR4Signal TransductionTuberculosis, PleuralYoung AdultConceptsSDF-1/CXCR4 axisStromal cell-derived factor-1SDF-1 levelsPleural fluidB cellsPeripheral bloodTuberculous pleuritisCXCR4 axisCell-derived factor-1Early secretory antigenic targetTuberculous pleural fluidMemory B cellsESAT-6 proteinField of tuberculosisB cell biologyTP patientsAntigenic targetsImmune responsePleural spaceB lymphocytesProtein 10TuberculosisM. tuberculosisMycobacterium tuberculosisMore antibodiesHuman natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12
Fu X, Liu Y, Li L, Li Q, Qiao D, Wang H, Lao S, Fan Y, Wu C. Human natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12. Immunology 2011, 134: 41-49. PMID: 21711347, PMCID: PMC3173693, DOI: 10.1111/j.1365-2567.2011.03464.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAntigens, CDAntigens, Differentiation, T-LymphocyteCD56 AntigenCell CountCytotoxicity, ImmunologicFemaleGPI-Linked ProteinsGranzymesHumansInterferon-gammaInterleukin-12Interleukin-2 Receptor alpha SubunitK562 CellsKiller Cells, NaturalLectins, C-TypeLeukocyte Common AntigensLeukocytes, MononuclearLymphocyte ActivationLymphocyte SubsetsMaleMiddle AgedNK Cell Lectin-Like Receptor Subfamily CNK Cell Lectin-Like Receptor Subfamily KPleural EffusionReceptors, IgGReceptors, Interleukin-12Tuberculosis, PleuralYoung AdultConceptsPleural fluid cellsNatural killer cellsNK cellsKiller cellsInterleukin-12Human natural killer cellsAdaptive immune featuresCD45RO- NK cellsInnate immune lymphocytesHuman NK cellsIL-12 receptor β1Immune lymphocytesIL-12Tuberculous patientsTuberculous pleurisyImmune featuresGranzyme BMore interferonReceptor β1Mycobacterium tuberculosisCD45ROFluid cellsPresent studyCellsCD25Biological functions of Mycobacterium tuberculosis-specific CD4+T cells were impaired by tuberculosis pleural fluid
Li Q, Li L, Liu Y, Fu X, Wang H, Lao S, yang B, Wu C. Biological functions of Mycobacterium tuberculosis-specific CD4+T cells were impaired by tuberculosis pleural fluid. Immunology Letters 2011, 138: 113-121. PMID: 21549757, DOI: 10.1016/j.imlet.2011.03.008.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAmino Acid SequenceAntibodies, NeutralizingAntigens, BacterialBacterial ProteinsBody FluidsCD4-Positive T-LymphocytesCells, CulturedFemaleHumansInterferon-gammaInterleukin-10Interleukin-2LungLymphocyte ActivationMaleMiddle AgedMolecular Sequence DataMonocytesMycobacterium tuberculosisTransforming Growth Factor betaTuberculosis, PleuralTumor Necrosis Factor-alphaConceptsPleural fluid mononuclear cellsPleural fluidAntigen-specific cellular immune responsesFluid mononuclear cellsIFN-γ productionMycobacterium tuberculosis infectionCellular immune responsesProduction of IFNT cell functionDose-dependent mannerTuberculosis patientsIL-10Tuberculosis infectionTuberculosis pleurisyCytokine productionMononuclear cellsIL-2Activation moleculeImmune responseCell activation processCFSE labelingCell activationFunctional predominanceCell activityStrong inhibitory effectPleural fluid from tuberculous pleurisy inhibits the functions of T cells and the differentiation of Th1 cells via immunosuppressive factors
Li Q, Li L, Liu Y, Fu X, Qiao D, Wang H, Lao S, Huang F, Wu C. Pleural fluid from tuberculous pleurisy inhibits the functions of T cells and the differentiation of Th1 cells via immunosuppressive factors. Cellular & Molecular Immunology 2011, 8: 172-180. PMID: 21258363, PMCID: PMC4003141, DOI: 10.1038/cmi.2010.80.Peer-Reviewed Original ResearchConceptsT cell functionT cellsIL-10Tuberculosis pleurisyTh1 cellsLocal immune statusCourse of diseaseTumor necrosis factorTh1 cell differentiationGrowth factor-β1Inhibitor of indoleamineImmunosuppressive mediatorsEffect of PFImmunosuppressive factorsCytokine productionImmune statusTuberculous pleurisyPleural fluidImmunopathological factorsFactor-β1Cell activationPleurisyGrowth factorCell cycle progressionCycle progression