2013
Overexpression of Pendrin in Intercalated Cells Produces Chloride-Sensitive Hypertension
Jacques T, Picard N, Miller RL, Riemondy KA, Houillier P, Sohet F, Ramakrishnan SK, Büsst CJ, Jayat M, Cornière N, Hassan H, Aronson PS, Hennings JC, Hübner CA, Nelson RD, Chambrey R, Eladari D. Overexpression of Pendrin in Intercalated Cells Produces Chloride-Sensitive Hypertension. Journal Of The American Society Of Nephrology 2013, 24: 1104-1113. PMID: 23766534, PMCID: PMC3699825, DOI: 10.1681/asn.2012080787.Peer-Reviewed Original ResearchConceptsDistal nephronTransgenic miceSame sodium intakeHigh salt intakeCause of hypertensionHigh-salt dietSodium-driven chloride/bicarbonate exchangerNet NaCl absorptionArterial hypertensionSalt intakeSodium intakeEpithelial sodium channel ENaCPathogenic roleNormal miceHypertensionRenal absorptionExchanger pendrinPrimary abnormalityVascular volumeAppropriate downregulationIntercalated cellsChloride absorptionSodium channel ENaCActivity of transportersPrimary activation
2010
The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice
Leviel F, Hübner CA, Houillier P, Morla L, Moghrabi S, Brideau G, Hatim H, Parker MD, Kurth I, Kougioumtzes A, Sinning A, Pech V, Riemondy KA, Miller RL, Hummler E, Shull GE, Aronson PS, Doucet A, Wall SM, Chambrey R, Eladari D. The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice. Journal Of Clinical Investigation 2010, 120: 1627-1635. PMID: 20389022, PMCID: PMC2860930, DOI: 10.1172/jci40145.Peer-Reviewed Original ResearchConceptsNa-Cl cotransporterSodium transportMaintenance of euvolemiaTransepithelial NaCl absorptionDucts of miceEpithelial sodium channelIntravascular volumeIndependent Cl-/HCO3Sodium balanceMouse CCDAmiloride-sensitive epithelial sodium channelSodium absorptionExcretion resultsFluid homeostasisGenetic ablationNaCl absorptionSodium channelsMiceCl-/HCO3NaCl transportSlc4a8Genetic disruptionNovel roleHydrochlorothiazideDuct
2006
Pendrin Regulation in Mouse Kidney Primarily Is Chloride-Dependent
Vallet M, Picard N, Loffing-Cueni D, Fysekidis M, Bloch-Faure M, Deschênes G, Breton S, Meneton P, Loffing J, Aronson PS, Chambrey R, Eladari D. Pendrin Regulation in Mouse Kidney Primarily Is Chloride-Dependent. Journal Of The American Society Of Nephrology 2006, 17: 2153-2163. PMID: 16825334, DOI: 10.1681/asn.2005101054.Peer-Reviewed Original ResearchConceptsHydrochlorothiazide administrationAldosterone secretionExtracellular fluid volume regulationElevated plasma aldosterone levelsPlasma aldosterone levelsDifferent mouse modelsFluid volume regulationPendrin protein expressionAldosterone levelsSecondary hyperaldosteronismEpithelial sodium channelChloride transportApical Cl-/HCO3Chloride reabsorptionRat modelPendrin expressionMouse modelKnockout miceSemiquantitative immunoblottingNaCl loadingGamma-ENaC subunitsBiologic effectsExperimental modelSodium channelsProtein expression
1999
Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice
Igarashi P, Shashikant C, Thomson R, Whyte D, Liu-Chen S, Ruddle F, Aronson P. Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice. American Journal Of Physiology 1999, 277: f599-f610. PMID: 10516285, DOI: 10.1152/ajprenal.1999.277.4.f599.Peer-Reviewed Original ResearchConceptsKsp-cadherinEscherichia coli lacZ reporter geneTissue-specific memberKsp-cadherin gene promoterRepeat-induced geneTranscriptional start siteTissue-specific expressionDifferentiation-specific expressionBeta-galactosidase enzyme activityTransgene integration sitesKidney-specific expressionLacZ reporter geneTransgenic miceEpithelial cellsKidney-specific activityStart siteRegulatory elementsGene promoterReporter genePronuclear microinjectionKb upstreamUreteric budTubular epithelial cellsDevelopmental expressionIntegration sites