2007
Ontogeny of NHE8 in the rat proximal tubule
Becker AM, Zhang J, Goyal S, Dwarakanath V, Aronson PS, Moe OW, Baum M. Ontogeny of NHE8 in the rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 293: f255-f261. PMID: 17429030, PMCID: PMC4119019, DOI: 10.1152/ajprenal.00400.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternDNA, ComplementaryElectrophoresis, Polyacrylamide GelFemaleImmunohistochemistryKidney Tubules, ProximalMicroscopy, FluorescenceMicrovilliPregnancyProteinsRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNASodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsRat proximal tubuleProximal tubulesAdult ratsNHE3 expressionProximal tubule bicarbonate reabsorptionReal-time RT-PCRProton secretionNeonatal proximal tubuleNHE8 expressionApical membraneBicarbonate reabsorptionMaturational increaseMaturational changesNeonatesNHE8RT-PCRBrush border membrane vesiclesTubulesRatsCortical membrane proteinsSecretionPresent studyDevelopmental expressionAdultsBorder membrane vesiclesNHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro
2004
Immunolocalization of NHE8 in rat kidney
Goyal S, Mentone S, Aronson PS. Immunolocalization of NHE8 in rat kidney. American Journal Of Physiology. Renal Physiology 2004, 288: f530-f538. PMID: 15522984, DOI: 10.1152/ajprenal.00229.2004.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityBiotinChlorocebus aethiopsCOS CellsElectrophoresis, Polyacrylamide GelFluorescent Antibody Technique, DirectHybridomasImmunohistochemistryKidneyKidney Tubules, ProximalMembranesMiceMice, KnockoutMicrovilliMolecular Sequence DataRatsRats, Sprague-DawleySodium-Hydrogen ExchangersRole of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells
Girardi AC, Knauf F, Demuth HU, Aronson PS. Role of dipeptidyl peptidase IV in regulating activity of Na+/H+ exchanger isoform NHE3 in proximal tubule cells. American Journal Of Physiology - Cell Physiology 2004, 287: c1238-c1245. PMID: 15213057, DOI: 10.1152/ajpcell.00186.2004.Peer-Reviewed Original ResearchConceptsProximal tubule cellsDPPIV inhibitorsDipeptidyl peptidase IVTubule cellsNHE3 activityOpossum kidney proximal tubule cellsKidney proximal tubule cellsNHE3 surface expressionPeptidase IVAdditive inhibitory effectEffects of genisteinForskolin/IBMXTyrosine kinase inhibitor genisteinSpecific competitive inhibitorPKA inhibitor HKinase inhibitor genisteinP32/98Activation of PKAInhibitory effectRenal brush border membraneBrush border membraneSurface expressionProtein kinase pathwayNHE3Inhibitor genistein
2000
Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*
Biemesderfer D, DeGray B, Aronson P. Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*. Journal Of Biological Chemistry 2000, 276: 10161-10167. PMID: 11120742, DOI: 10.1074/jbc.m008098200.Peer-Reviewed Original ResearchAcridine OrangeAnimalsAntibodiesCarrier ProteinsCell MembraneCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelEndosomesFluorescent Antibody Technique, IndirectGoatsHeymann Nephritis Antigenic ComplexHydrogenHydrogen-Ion ConcentrationImmunoblottingImmunohistochemistryKidneyKidney TubulesMagnesiumMaleMembrane GlycoproteinsMicrofilament ProteinsMicroscopy, ElectronMicrovilliMitochondriaPrecipitin TestsProtein BindingProtein Structure, TertiaryRabbitsRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSucroseTime Factors
1991
Increased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells
Reilly RF, Haggerty JG, Aronson PS, Adelberg EA, Slayman CW. Increased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells. American Journal Of Physiology 1991, 260: c738-c744. PMID: 1673319, DOI: 10.1152/ajpcell.1991.260.4.c738.Peer-Reviewed Original Research