2006
The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates
Knauf F, Mohebbi N, Teichert C, Herold D, Rogina B, Helfand S, Gollasch M, Luft FC, Aronson PS. The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates. Biochemical Journal 2006, 397: 25-29. PMID: 16608441, PMCID: PMC1479758, DOI: 10.1042/bj20060409.Peer-Reviewed Original ResearchConceptsExternal citrateKrebs cycle intermediatesXenopus oocytesSodium dicarboxylate cotransporterMammalian genesDrosophila melanogasterIndy mutantsLifespan extensionLongevity genesPlasma membraneSubstrate effluxExternal mediumSpecific transport mechanismsFunctional studiesSuccinate uptakeAddition of succinateEnergy metabolismIndyTransportable substratesGenesOocytesEffluxMelanogasterMutantsSuccinate
1999
Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice
Igarashi P, Shashikant C, Thomson R, Whyte D, Liu-Chen S, Ruddle F, Aronson P. Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice. American Journal Of Physiology 1999, 277: f599-f610. PMID: 10516285, DOI: 10.1152/ajprenal.1999.277.4.f599.Peer-Reviewed Original ResearchConceptsKsp-cadherinEscherichia coli lacZ reporter geneTissue-specific memberKsp-cadherin gene promoterRepeat-induced geneTranscriptional start siteTissue-specific expressionDifferentiation-specific expressionBeta-galactosidase enzyme activityTransgene integration sitesKidney-specific expressionLacZ reporter geneTransgenic miceEpithelial cellsKidney-specific activityStart siteRegulatory elementsGene promoterReporter genePronuclear microinjectionKb upstreamUreteric budTubular epithelial cellsDevelopmental expressionIntegration sites
1997
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. American Journal Of Physiology 1997, 273: f289-f299. PMID: 9277590, DOI: 10.1152/ajprenal.1997.273.2.f289.Peer-Reviewed Original ResearchConceptsProximal tubulesRat kidneyMonoclonal antibodiesLoop of HenleHigh-resolution immunocytochemical studiesProximal tubule cellsPrevious immunochemical studiesIsoform-specific monoclonal antibodiesNeonatal rat kidneyTubule cellsImmunocytochemical studyThick limbImmunocytochemical techniquesKidneyLAP cellsRenal epitheliumNHE3Immunochemical studiesHigh-resolution immunocytochemical techniqueAdultsLater stagesNephron development
1995
Ontogeny of rabbit renal cortical NHE3 and NHE1: effect of glucocorticoids
Baum M, Biemesderfer D, Gentry D, Aronson PS. Ontogeny of rabbit renal cortical NHE3 and NHE1: effect of glucocorticoids. American Journal Of Physiology 1995, 268: f815-f820. PMID: 7771509, DOI: 10.1152/ajprenal.1995.268.5.f815.Peer-Reviewed Original ResearchConceptsProximal tubulesNHE1 mRNABicarbonate absorptionNHE3 mRNA abundanceDaily subcutaneous administrationAdult animalsAdministration of glucocorticoidsEffects of glucocorticoidsNHE3 protein abundanceNeonatal proximal tubuleDays of ageRenal maturationSubcutaneous administrationRenal cortexNeonatal rabbitsCortical developmentProtein abundanceRate of maturationGlucocorticoidsNHE3 mRNAAntiporter activityApical membraneNHE3Lower ratesTubules
1992
Assembly of distinctive coated pit and microvillar microdomains in the renal brush border
Biemesderfer D, Dekan G, Aronson PS, Farquhar MG. Assembly of distinctive coated pit and microvillar microdomains in the renal brush border. American Journal Of Physiology 1992, 262: f55-f67. PMID: 1733297, DOI: 10.1152/ajprenal.1992.262.1.f55.Peer-Reviewed Original Research