2007
Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells
Zhang J, Bobulescu IA, Goyal S, Aronson PS, Baum MG, Moe OW. Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells. American Journal Of Physiology. Renal Physiology 2007, 293: f761-f766. PMID: 17581925, PMCID: PMC2861566, DOI: 10.1152/ajprenal.00117.2007.Peer-Reviewed Original ResearchConceptsNRK cellsBasolateral NHE activityNHE8 proteinApical membraneNHE1 protein levelsPrimary amino acid sequenceAmino acid sequenceProtein levelsProtein expressionNHE8 protein expressionBasolateral membraneCultured renal epithelial cellsRenal epithelial cellsMammalian cellsNHE activityFunctional characterizationPlasma membraneAcid sequenceRenal cell linesNHE1 protein expressionNative environmentImmunoelectron microscopyExchange activityCell linesNHE8
2003
Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD
Thomson RB, Mentone S, Kim R, Earle K, Delpire E, Somlo S, Aronson PS. Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. American Journal Of Physiology. Renal Physiology 2003, 285: f870-f880. PMID: 12851251, DOI: 10.1152/ajprenal.00153.2003.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsCadherinsDisease Models, AnimalEpitheliumGene SilencingImmunohistochemistryKidneyMiceMicroscopy, ElectronMicroscopy, Electron, ScanningPolycystic Kidney, Autosomal DominantProtein Kinase D2Protein KinasesSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseSolute Carrier Family 12, Member 2Tissue DistributionConceptsCell polarityKsp-cadherinBasolateral membraneRenal cystic epitheliaEpithelial cell polarityEpithelial cellsSpecific membrane proteinsCell dedifferentiationEpithelial cell dedifferentiationE-cadherinRenal epithelial cellsCystic epithelial cellsMembrane proteinsPhenotypic transitionCl(-) cotransporter NKCC1Expression levelsATPaseSecretory isoformEarly cystsTransepithelial secretionImmunocytochemical analysisUltrastructural analysisAutosomal dominant polycystic kidney diseaseDominant polycystic kidney diseasePolycystic kidney disease
1999
Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte D, Li C, Thomson R, Nix S, Zanjani R, Karp S, Aronson P, Igarashi P. Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity. American Journal Of Physiology 1999, 277: f587-f598. PMID: 10516284, DOI: 10.1152/ajprenal.1999.277.4.f587.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 3Activator protein-2Renal epithelial cellsCAAT boxNuclear proteinsFlanking regionsPromoter activityTissue-specific gene expressionEpithelial cellsElectrophoretic mobility shift assaysKsp-cadherin gene promoterCCAAT/enhancer-binding proteinTranscription initiation siteKidney-specific memberMobility shift assaysConsensus promoter elementsEnhancer-binding proteinCell-specific activityPotential binding sitesLambda bacteriophage clonesLuciferase reporter plasmidHelix proteinsKidney epithelial cellsGATA factorsGC box
1991
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1Molecular biology of renal Na(+)-H+ exchangers.
Igarashi P, Reilly RF, Hildebrandt F, Biemesderfer D, Rebouças NA, Slayman CW, Aronson PS. Molecular biology of renal Na(+)-H+ exchangers. Kidney International Supplement 1991, 33: s84-9. PMID: 1653876.Commentaries, Editorials and LettersConceptsKb cDNALLC-PK1 cellsSteady-state transcript levelsPlasma membrane proteinsMembrane-associated domainSequence of cDNACDNA library screeningAmino acid sequenceExchanger gene expressionNorthern blot analysisEvolutionary conservationRenal epithelial cellsCytoplasmic domainMembrane proteinsRegulation of intracellularAcid sequenceTranscript levelsUntranslated regionGene expressionSimilar transcriptsCDNALibrary screeningMolecular biologyPolymerase chain reactionBlot analysis