2007
Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells
Zhang J, Bobulescu IA, Goyal S, Aronson PS, Baum MG, Moe OW. Characterization of Na+/H+ exchanger NHE8 in cultured renal epithelial cells. American Journal Of Physiology. Renal Physiology 2007, 293: f761-f766. PMID: 17581925, PMCID: PMC2861566, DOI: 10.1152/ajprenal.00117.2007.Peer-Reviewed Original ResearchConceptsNRK cellsBasolateral NHE activityNHE8 proteinApical membraneNHE1 protein levelsPrimary amino acid sequenceAmino acid sequenceProtein levelsProtein expressionNHE8 protein expressionBasolateral membraneCultured renal epithelial cellsRenal epithelial cellsMammalian cellsNHE activityFunctional characterizationPlasma membraneAcid sequenceRenal cell linesNHE1 protein expressionNative environmentImmunoelectron microscopyExchange activityCell linesNHE8Ontogeny of NHE8 in the rat proximal tubule
Becker AM, Zhang J, Goyal S, Dwarakanath V, Aronson PS, Moe OW, Baum M. Ontogeny of NHE8 in the rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 293: f255-f261. PMID: 17429030, PMCID: PMC4119019, DOI: 10.1152/ajprenal.00400.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternDNA, ComplementaryElectrophoresis, Polyacrylamide GelFemaleImmunohistochemistryKidney Tubules, ProximalMicroscopy, FluorescenceMicrovilliPregnancyProteinsRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNASodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsRat proximal tubuleProximal tubulesAdult ratsNHE3 expressionProximal tubule bicarbonate reabsorptionReal-time RT-PCRProton secretionNeonatal proximal tubuleNHE8 expressionApical membraneBicarbonate reabsorptionMaturational increaseMaturational changesNeonatesNHE8RT-PCRBrush border membrane vesiclesTubulesRatsCortical membrane proteinsSecretionPresent studyDevelopmental expressionAdultsBorder membrane vesicles
2002
Renal expression of novel Na+/H+exchanger isoform NHE8
Goyal S, Heuvel G, Aronson PS. Renal expression of novel Na+/H+exchanger isoform NHE8. American Journal Of Physiology. Renal Physiology 2002, 284: f467-f473. PMID: 12409279, DOI: 10.1152/ajprenal.00352.2002.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibody SpecificityCation Transport ProteinsCOS CellsDrosophila ProteinsHumansImmunoblottingIn Situ HybridizationKidneyMembrane ProteinsMiceMicrovilliMolecular Sequence DataOrgan SpecificityProtein IsoformsRatsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersTransfectionConceptsProximal tubulesComplete open reading frameProximal tubule localizationRat renal cortexAmino acid identityOpen reading frameRenal expressionCortical tubulesRenal cortexOuter stripeOuter medullaNull miceMammalian orthologsWestern blotDrosophila melanogasterMouse kidneyMedullaMembrane ion transportProtein expressionAcid identityNHE8CortexMammalian isoformsReading frameNHE isoforms