2005
Identification of Binding Sites of EVI1 in Mammalian Cells*
Yatsula B, Lin S, Read AJ, Poholek A, Yates K, Yue D, Hui P, Perkins AS. Identification of Binding Sites of EVI1 in Mammalian Cells*. Journal Of Biological Chemistry 2005, 280: 30712-30722. PMID: 16006653, DOI: 10.1074/jbc.m504293200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesDNADNA-Binding ProteinsHerpes Simplex Virus Protein Vmw65HumansMDS1 and EVI1 Complex Locus ProteinMiceMolecular Sequence DataMutagenesis, Site-DirectedMutation, MissenseNIH 3T3 CellsOligonucleotide Array Sequence AnalysisProtein ConformationProto-OncogenesRecombinant Fusion ProteinsTranscription FactorsZinc FingersConceptsChromatin immunoprecipitationTarget genesN-terminal DNAPutative target genesVP16 fusion proteinTranscription start siteN-terminal domainGel shift assaysNIH 3T3 cellsZFPM2/FOG2Transcriptional activatorEndogenous genesMissense mutantsEVI1 bindsZinc fingerMammalian cellsStart siteShift assaysMutant formsFusion proteinTransactivation studiesSequence analysisGenesEVI1Binding sites
1998
Identification of candidate target genes for EVI-1, a zinc finger oncoprotein, using a novel selection strategy
Kim J, Hui P, Yue D, Aycock J, Leclerc C, Bjoring A, Perkins A. Identification of candidate target genes for EVI-1, a zinc finger oncoprotein, using a novel selection strategy. Oncogene 1998, 17: 1527-1538. PMID: 9794230, DOI: 10.1038/sj.onc.1202331.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBase SequenceBinding SitesCalcium ChannelsDNA-Binding ProteinsDNA, ComplementaryEscherichia coliExonsGenomic LibraryHaploidyHumansInositol 1,4,5-Trisphosphate ReceptorsMDS1 and EVI1 Complex Locus ProteinMiceMolecular Sequence DataOncogene ProteinsProto-OncogenesReceptors, Cytoplasmic and NuclearRecombinant Fusion ProteinsSequence AlignmentTranscription FactorsTumor Cells, CulturedZinc FingersConceptsGenomic fragmentEvi-1Target genesZinc finger proteinHybrid selectionCandidate target genesTetracycline-regulated systemChimeric activatorFinger proteinHaploid genomeMouse cDNAMouse genomeGene sequencesMouse DNAFusion proteinTwo-step selectionGenesGenomeProteinCDNANovel selection strategyFragmentsHigh affinitySublibrariesITPR2