Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo
Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 18206-18211. PMID: 15608064, PMCID: PMC539748, DOI: 10.1073/pnas.0407976101.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesElectrophysiologyGenetic VectorsGreen Fluorescent ProteinsHippocampusImage Processing, Computer-AssistedLentivirusMiceMice, Inbred C57BLMicroscopyNeuronsPhenotypePhotonsPromoter Regions, GeneticRecombinant ProteinsRNA, Small InterferingRNA, Small NuclearSynapsinsConceptsU6 promoter-driven expressionMicroscopy-based techniquesGenetic manipulationGene deliveryLentiviral vectorsGene functionGene knockdownSubsequent phenotypic analysisPromoter-driven expressionCellular response propertiesSmall networksTwo-photon microscopyTwo-photon time-lapse imagingCortical neuronsEfficient meansMicroscopyAlpha-calcium/calmodulin-dependent protein kinase IIThe transcription factor orthodenticle homeobox 2 influences axonal projections and vulnerability of midbrain dopaminergic neurons
Chung CY, Licznerski P, Alavian KN, Simeone A, Lin Z, Martin E, Vance J, Isacson O. The transcription factor orthodenticle homeobox 2 influences axonal projections and vulnerability of midbrain dopaminergic neurons. Brain 2010, 133: 2022-2031. PMID: 20573704, PMCID: PMC2892944, DOI: 10.1093/brain/awq142.Peer-Reviewed Original ResearchConceptsMidbrain dopaminergic neuronsVentral mesencephalic culturesTranscription factor orthodenticle homeobox 2Orthodenticle homeobox 2Dopaminergic neuronsMesencephalic culturesShort hairpin RNAHomeobox 2A10 dopaminergic neuronsCyclase-activating peptideHairpin RNAConditional knockout miceVentral mesencephalonNeuronal vulnerabilityDopaminergic projectionsAxonal projectionsParkinson's diseaseAdult miceKnockout miceMN9D cellsNeuropilin-2Elevated geneNeuronsNeuropilin-1Human midbrain