Bioengineered lungs generated from human iPSCs‐derived epithelial cells on native extracellular matrix
Ghaedi M, Le AV, Hatachi G, Beloiartsev A, Rocco K, Sivarapatna A, Mendez JJ, Baevova P, Dyal RN, Leiby KL, White ES, Niklason LE. Bioengineered lungs generated from human iPSCs‐derived epithelial cells on native extracellular matrix. Journal Of Tissue Engineering And Regenerative Medicine 2017, 12: e1623-e1635. PMID: 29024475, PMCID: PMC5991621, DOI: 10.1002/term.2589.Peer-Reviewed Original ResearchConceptsEpithelial progenitor cellsProgenitor cellsStem cellsEpithelial cellsLung progenitor cellsLung epithelial cellsHuman lungHuman-derived cellsReliable cell sourceNative extracellular matrixEarly stepsExtracellular matrixRat lungImportant early stepHuman iPSCLung regenerationLung tissue regenerationHuman lung tissueIPSCsLung scaffoldsCell sourceCellsEpithelial markersLung transplantationTissue regenerationEfficient and Functional Endothelial Repopulation of Whole Lung Organ Scaffolds
Le AV, Hatachi G, Beloiartsev A, Ghaedi M, Engler AJ, Baevova P, Niklason LE, Calle EA. Efficient and Functional Endothelial Repopulation of Whole Lung Organ Scaffolds. ACS Biomaterials Science & Engineering 2017, 3: 2000-2010. PMID: 33440555, DOI: 10.1021/acsbiomaterials.6b00784.Peer-Reviewed Original ResearchLong-term functionNative lungBarrier functionRat lungEndothelial cellsEndothelial nitric oxide synthaseLung microvascular endothelial cellsNitric oxide synthaseTissue plasminogen activatorVascular surface areaExposure of bloodMicrovascular endothelial cellsGas exchange functionRat lung microvascular endothelial cellsMechanical ventilationPulmonary arteryPulmonary vasculatureImproved barrier functionOxide synthaseVascular pressuresLung tissueFunctional endotheliumVE-cadherin gene expressionAntithrombotic substancesLung