881. Saliva-Based, Extraction-Free PCR Testing For The Detection Of Key Respiratory Pathogens
Allicock O, Lin T, Fajardo K, Yolda-Carr D, Hislop M, Wang J, Zuniga D, Platt W, Tuohy B, Peno C, Wyllie A. 881. Saliva-Based, Extraction-Free PCR Testing For The Detection Of Key Respiratory Pathogens. Open Forum Infectious Diseases 2023, 10: ofad500.926. PMCID: PMC10677551, DOI: 10.1093/ofid/ofad500.926.Peer-Reviewed Original ResearchSARS-CoV-2Saliva samplesRespiratory virusesRespiratory pathogensSARS-CoV-2 PCR testInfluenza A/BSARS-CoV-2 testingUpper respiratory tract pathogensAdditional respiratory virusesDetection of hMPVMass testing strategyPCR testCommon respiratory virusesCommon respiratory pathogensRespiratory tract pathogensKey respiratory pathogensYears of ageSARS-CoV-2 detectionGrant/research supportTransmissible infectious diseasesUse of salivaYale HealthRespiratory symptomsInfluenza B.Nasopharyngeal swabsPooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2
Allicock O, Yolda-Carr D, Todd J, Wyllie A. Pooled RNA-extraction-free testing of saliva for the detection of SARS-CoV-2. Scientific Reports 2023, 13: 7426. PMID: 37156888, PMCID: PMC10165292, DOI: 10.1038/s41598-023-34662-2.Peer-Reviewed Original ResearchConceptsSARS-CoV-2RT-qPCR assaysSARS-CoV-2 testingVirus-infected individualsAged care facilitiesSelf-collected salivaSARS-CoV-2 spreadActionable time frameAsymptomatic individualsTest turnaround timeHealthcare workersGeneral populationCare facilitiesTest availabilityWeekly testingFree testingClinical salivaPositive agreementClinical laboratoriesSalivaCT valuesSuch testingTesting workflowIndividual testingTesting protocol