2001
A triple color FISH technique for mouse chromosome identification
Henegariu O, Dunai J, Chen X, Korenberg J, Ward D, Greally J. A triple color FISH technique for mouse chromosome identification. Mammalian Genome 2001, 12: 462-465. PMID: 11353394, DOI: 10.1007/s003350020038.Peer-Reviewed Original ResearchAnimalsCell LineChromosome MappingChromosomesChromosomes, Artificial, BacterialIn Situ Hybridization, FluorescenceKaryotypingMiceTransgenesTranslocation, GeneticMulti-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell
Krause D, Theise N, Collector M, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis S. Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell. Cell 2001, 105: 369-377. PMID: 11348593, DOI: 10.1016/s0092-8674(01)00328-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CD34Antigens, LyBone Marrow CellsCell LineageCell MovementEpithelial CellsFemaleFluorescent DyesHematopoietic Stem Cell TransplantationHematopoietic Stem CellsHumansImmunohistochemistryIn Situ Hybridization, FluorescenceIntestine, SmallKeratinsLungMaleMembrane ProteinsMiceMice, KnockoutOrganic ChemicalsPulmonary SurfactantsStem CellsY ChromosomeConceptsLong-term repopulationSingle bone marrowMulti-lineage engraftmentAdult bone marrow cellsProperties of HSCHematopoietic stemSecondary hostsGenetic diseasesStem cellsBone marrow cellsExpression increasesDifferentiative capacityBone marrowEpithelial cellsSerial transplantationRare cellsTissue repairMarrow cellsCellsDifferentiationHostSecondary recipientsGI tractPhenotypeMarrowSmall Marker Chromosome Identification in Metaphase and Interphase Using Centromeric Multiplex FISH (CM-FISH)
Henegariu O, Bray-Ward P, Artan S, Vance G, Qumsyieh M, Ward D. Small Marker Chromosome Identification in Metaphase and Interphase Using Centromeric Multiplex FISH (CM-FISH). Laboratory Investigation 2001, 81: 475-481. PMID: 11304566, DOI: 10.1038/labinvest.3780255.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyCell NucleusCentromereChromosome AberrationsChromosome DisordersChromosomes, HumanDNA, SatelliteGenetic MarkersHumansIn Situ Hybridization, FluorescenceInterphaseKaryotypingMetaphaseConceptsHuman chromosome painting probesMolecular cytogenetic proceduresDNA probesSubtelomeric chromosomal regionsIdentification of aneuploidyChromosome painting probesM-FISH analysisAlpha-satellite regionMarker chromosome identificationMarker chromosomesChromosome identificationHuman chromosomesRepetitive sequencesChromosomal rearrangementsInterphase nucleiMultiplex FISHChromosomal regionsPainting probesMetaphase assayMultiplex fluorescenceM-FISHCytogenetic proceduresSpectral karyotypingChromosomesPlasmid probesCryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH (TM-FISH) Strategies
Henegariu O, Artan S, Greally J, Chen X, Korenberg J, Vance G, Stubbs L, Bray-Ward P, Ward D. Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH (TM-FISH) Strategies. Laboratory Investigation 2001, 81: 483-491. PMID: 11304567, DOI: 10.1038/labinvest.3780256.Peer-Reviewed Original ResearchConceptsSubtelomeric regionsHuman subtelomeric regionsMouse chromosomesHuman chromosomesP1 clonesMouse probeMultiplex fluorescenceM-FISHChromosomesFISH strategySet of probesStandard fluorescence microscopeSmall rearrangementsSitu hybridizationLabeling strategyCryptic translocationSubtelomeric probesIdiopathic mental retardationFluorescence microscopeKnown markerCentromeresKilobasesImprovements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing
Henegariu O, Heerema N, Wright L, Bray‐Ward P, Ward D, Vance G. Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 2001, 43: 101-109. PMID: 11169574, DOI: 10.1002/1097-0320(20010201)43:2<101::aid-cyto1024>3.0.co;2-8.Peer-Reviewed Original ResearchMeSH KeywordsCell LineCentrifugationChromosome BandingChromosome PaintingDNADNA, NeoplasmHumansIn Situ Hybridization, FluorescenceNucleic Acid DenaturationTumor Cells, CulturedConceptsComparative genomic hybridizationClassical banding techniquesSitu hybridization applicationsChromosomal integrityChromosome spreadsMultiplex FISHMolecular cytogeneticsFISH applicationsChromosome spreadingFISH techniqueGenomic hybridizationEnvironmental conditionsSitu hybridizationBanding techniquesChromosomesHybridizationReproducible protocolFISH resultsCytogenetic slidesCell suspensionsSlide preparationFishMetaphase
2000
Liver from bone marrow in humans
Theise N, Nimmakayalu M, Gardner R, Illei P, Morgan G, Teperman L, Henegariu O, Krause D. Liver from bone marrow in humans. Hepatology 2000, 32: 11-16. PMID: 10869283, DOI: 10.1053/jhep.2000.9124.Peer-Reviewed Original ResearchAdultBone Marrow CellsBone Marrow TransplantationFemaleHumansImmunohistochemistryIn Situ Hybridization, FluorescenceLiverLiver TransplantationMaleMiddle AgedStem CellsCustom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling
Henegariu O, Bray-Ward P, Ward D. Custom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling. Nature Biotechnology 2000, 18: 345-348. PMID: 10700155, DOI: 10.1038/73815.Peer-Reviewed Original ResearchMeSH KeywordsBiotinDigoxigeninFluorescent DyesHumansIn Situ Hybridization, FluorescenceKaryotypingNucleotidesConceptsSuccinimidyl ester derivativeNucleic acid hybridization assaysNucleic acid labelingLabeling reactionCustom preparationsUseful dyesFluorescent dyeDyeAcid labelingFluorescent nucleotidesCommercial sourcesInexpensive procedureSynthesisDerivativesReactionLaboratory applicationsHybridization assaysPreparationBiotinFluorescentDerivation of hepatocytes from bone marrow cells in mice after radiation‐induced myeloablation
Theise N, Badve S, Saxena R, Henegariu O, Sell S, Crawford J, Krause D. Derivation of hepatocytes from bone marrow cells in mice after radiation‐induced myeloablation. Hepatology 2000, 31: 235-240. PMID: 10613752, DOI: 10.1002/hep.510310135.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowBone Marrow CellsBone Marrow TransplantationFemaleFlow CytometryIn Situ Hybridization, FluorescenceLiverMaleMiceStem CellsWhole-Body IrradiationY ChromosomeConceptsBone marrow cellsY chromosomeMarrow cellsFemale miceMessenger RNAWhole bone marrow transplantsAge-matched male donorsDerivation of hepatocytesSkeletal muscle regenerationSevere acute injuryAcute hepatic injuryBone marrow transplantationBone marrow transplantSimultaneous FISHFluorescence-activated cell sorterMale-derived cellsOval cell proliferationDays posttransplantationMonths posttransplantationHepatic injuryMarrow transplantationMarrow transplantAcute injuryMonth 2Hepatic engraftment
1999
Colour-changing karyotyping: an alternative to M-FISH/SKY
Henegariu O, Heerema N, Bray-Ward P, Ward D. Colour-changing karyotyping: an alternative to M-FISH/SKY. Nature Genetics 1999, 23: 263-264. PMID: 10545937, DOI: 10.1038/15437.Peer-Reviewed Original Research
1998
Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection
Blough R, Vance G, Henegariu O, Smolarek T, Sledge G, Heerema N. Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection. Cancer Genetics 1998, 106: 24-29. PMID: 9772905, DOI: 10.1016/s0165-4608(98)00042-9.Peer-Reviewed Original ResearchMeSH KeywordsChromosome AberrationsChromosomes, Human, Pair 12GerminomaHumansIn Situ Hybridization, FluorescenceMaleTesticular NeoplasmsTumor Cells, CulturedConceptsChromosome 12 regionDistal 12pNormal human chromosomesBeta-satellite DNABand-specific probesFISH experimentsChromosome arm 12pChromosome microdissectionChromosomal microdissectionHuman chromosomesPainting probesChromosome 12Stalk regionCisplatin-resistant subclonesSitu hybridizationRDNACommon breakpointFishMicrodissectionSpecific originChromatinRearrangementChromosomesUnusual distributionBreakpointsTriple-color FISH analysis of 12p amplification in testicular germ-cell tumors using 12p band-specific painting probes
Henegariu O, Vance G, Heiber D, Pera M, Heerema N. Triple-color FISH analysis of 12p amplification in testicular germ-cell tumors using 12p band-specific painting probes. Journal Of Molecular Medicine 1998, 76: 648-655. PMID: 9725767, DOI: 10.1007/s001090050262.Peer-Reviewed Original ResearchMolecular cytogenetic analysis of patients with holoprosencephaly and structural rearrangements of 7q
Vance G, Nickerson C, Sarnat L, Zhang A, Henegariu O, Morichon‐Delvallez N, Butler M, Palmer C. Molecular cytogenetic analysis of patients with holoprosencephaly and structural rearrangements of 7q. American Journal Of Medical Genetics 1998, 76: 51-57. PMID: 9508065, PMCID: PMC6775641, DOI: 10.1002/(sici)1096-8628(19980226)76:1<51::aid-ajmg9>3.0.co;2-s.Peer-Reviewed Original ResearchMeSH KeywordsCell LineChromosome AberrationsChromosome DeletionChromosomes, Human, Pair 13Chromosomes, Human, Pair 22Chromosomes, Human, Pair 7CytogeneticsFemaleGenetic MarkersHedgehog ProteinsHoloprosencephalyHumansIn Situ Hybridization, FluorescenceInfantInfant, NewbornKaryotypingMaleMosaicismPolymerase Chain ReactionProteinsTrans-ActivatorsTranslocation, GeneticConceptsSonic hedgehog geneHedgehog geneCell linesDifferent chromosome regionsStructural rearrangementsHuman Sonic Hedgehog genePatient cell linesMolecular cytogenetic analysisChromosome regionsChromosome 7Embryonic forebrainLong armGenesSitu hybridizationPolymerase chain reaction amplificationHPE3Chain reaction amplificationCytogenetic analysisMidline cleavageReaction amplificationRearrangementPhenotypeCritical regionLinesHybridization
1997
PCR and FISH analysis of a ring Y chromosome
Henegariu O, Kernek S, Keating M, Palmer C, Heerema N. PCR and FISH analysis of a ring Y chromosome. American Journal Of Medical Genetics 1997, 69: 171-176. PMID: 9056556, DOI: 10.1002/(sici)1096-8628(19970317)69:2<171::aid-ajmg11>3.0.co;2-i.Peer-Reviewed Original ResearchAbnormalities, MultipleHumansHypospadiasIn Situ Hybridization, FluorescenceInfantMaleMosaicismPolymerase Chain ReactionRing ChromosomesSequence Analysis, DNAY Chromosome