2022
Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies
Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon MD, Neugebauer KM, Tebaldi T, Halene S. Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies. Molecular Cell 2022, 82: 1107-1122.e7. PMID: 35303483, PMCID: PMC8988922, DOI: 10.1016/j.molcel.2022.02.025.Peer-Reviewed Original Research
2018
A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs
Wang Z, Gearhart MD, Lee YW, Kumar I, Ramazanov B, Zhang Y, Hernandez C, Lu AY, Neuenkirchen N, Deng J, Jin J, Kluger Y, Neubert TA, Bardwell VJ, Ivanova NB. A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs. Cell Stem Cell 2018, 22: 235-251.e9. PMID: 29337181, PMCID: PMC5797497, DOI: 10.1016/j.stem.2017.12.002.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationChromatinF-Box ProteinsHistonesHuman Embryonic Stem CellsHumansJumonji Domain-Containing Histone DemethylasesLysineMethylationMultiprotein ComplexesPolycomb Repressive Complex 1Polycomb Repressive Complex 2Promoter Regions, GeneticProtein DomainsProto-Oncogene ProteinsRepressor ProteinsConceptsEmbryonic stem cellsHuman embryonic stem cellsPolycomb domainsNon-canonical PRC1 complexesKey developmental lociNon-canonical complexesPolycomb group (PcG) proteinsE3 ubiquitin ligasesStem cell systemInitiation of differentiationDevelopmental lociPRC1 complexesRepressive chromatinRepressor functionPRC1.1 complexMesoderm lineageGroup proteinsCo-repressorAccessory subunitsDifferentiation programCanonical complexDevelopmental systemsC-terminusGene targetsN-terminus
2015
Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization
Neuenkirchen N, Englbrecht C, Ohmer J, Ziegenhals T, Chari A, Fischer U. Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization. The EMBO Journal 2015, 34: 1925-1941. PMID: 26069323, PMCID: PMC4547896, DOI: 10.15252/embj.201490350.Peer-Reviewed Original ResearchConceptsSMN complexAssembly machinerySm proteinsSpliceosomal U snRNPsDetailed molecular dissectionTrans-acting factorsSpontaneous conformational changesBiochemical reconstitutionPRMT5 complexU snRNPsProtein complexesMolecular dissectionProtein substratesProtein organizationIndividual proteinsConformational changesFaithful deliveryMode of actionRecombinant sourcesCoordinated actionBrownian machineMechanistic insightsSnRNPsProteinAssembly reaction
2012
Binding of the Heterogeneous Ribonucleoprotein K (hnRNP K) to the Epstein-Barr Virus Nuclear Antigen 2 (EBNA2) Enhances Viral LMP2A Expression
Gross H, Hennard C, Masouris I, Cassel C, Barth S, Stober-Grässer U, Mamiani A, Moritz B, Ostareck D, Ostareck-Lederer A, Neuenkirchen N, Fischer U, Deng W, Leonhardt H, Noessner E, Kremmer E, Grässer FA. Binding of the Heterogeneous Ribonucleoprotein K (hnRNP K) to the Epstein-Barr Virus Nuclear Antigen 2 (EBNA2) Enhances Viral LMP2A Expression. PLOS ONE 2012, 7: e42106. PMID: 22879910, PMCID: PMC3411732, DOI: 10.1371/journal.pone.0042106.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntibody SpecificityArginineCell LineDNAEpstein-Barr Virus InfectionsEpstein-Barr Virus Nuclear AntigensGene Expression Regulation, ViralHerpesvirus 4, HumanHeterogeneous-Nuclear Ribonucleoprotein KHumansImmunoprecipitationMethylationMolecular Sequence DataMultiprotein ComplexesMutant ProteinsPromoter Regions, GeneticProtein BindingProtein TransportRepetitive Sequences, Amino AcidSnRNP Core ProteinsTransfectionViral Matrix ProteinsViral ProteinsConceptsEpstein-Barr virus nuclear antigen 2Survival motor neuron (SMN) proteinHnRNP KHeterogeneous ribonucleoprotein KRG repeatsCellular proteinsSDMA-specific antibodyGel shift experimentsMotor neuron proteinHnRNP K.Cellular homologueCellular processesSequence motifsNp9 proteinsHuman endogenous retrovirus KArginine residuesNeuron proteinEBNA2 proteinShift experimentsLMP2A expressionNuclear antigen 2ProteinUnknown mechanismE. coliArginine-Glycine
2008
An Assembly Chaperone Collaborates with the SMN Complex to Generate Spliceosomal SnRNPs
Chari A, Golas MM, Klingenhäger M, Neuenkirchen N, Sander B, Englbrecht C, Sickmann A, Stark H, Fischer U. An Assembly Chaperone Collaborates with the SMN Complex to Generate Spliceosomal SnRNPs. Cell 2008, 135: 497-509. PMID: 18984161, DOI: 10.1016/j.cell.2008.09.020.Peer-Reviewed Original ResearchConceptsSpliceosomal small nuclear ribonucleoproteinsSMN complexSm proteinsProtein unitsCore domainMRNA processing machinerySmall nuclear ribonucleoproteinSnRNP formationAssembly chaperonesPRMT5 complexClamp loaderNuclear ribonucleoproteinProcessing machinerySnRNAMode of actionPIClnStructural studiesProteinComplexesEssential componentPRMT5ChaperonesSnRNPRibonucleoproteinMachineryDeciphering the assembly pathway of Sm‐class U snRNPs
Neuenkirchen N, Chari A, Fischer U. Deciphering the assembly pathway of Sm‐class U snRNPs. FEBS Letters 2008, 582: 1997-2003. PMID: 18348870, DOI: 10.1016/j.febslet.2008.03.009.Peer-Reviewed Original ResearchConceptsU snRNPsAssembly pathwayUridine-rich small nuclear ribonucleoproteinsProtein arginine methyltransferase 5SMN complex functionTrans-acting factorsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinCommon proteinsSurvival motor neuronSm classSnRNPsProteinPathwayMotor neuronsRibonucleoproteinRecent progressRNASMNAssemblyLarge numberVitroVivoComplexes
2006
A Comprehensive Interaction Map of the Human Survival of Motor Neuron (SMN) Complex*
Otter S, Grimmler M, Neuenkirchen N, Chari A, Sickmann A, Fischer U. A Comprehensive Interaction Map of the Human Survival of Motor Neuron (SMN) Complex*. Journal Of Biological Chemistry 2006, 282: 5825-5833. PMID: 17178713, DOI: 10.1074/jbc.m608528200.Peer-Reviewed Original ResearchConceptsMotor neurons (SMN) complexSMN complexComprehensive interaction mapInteraction mapSm proteinsSMN proteinSpinal muscular atrophy (SMA) disease geneRich small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinAssembly machineryU snRNPsProtein SMNU snRNAsNuclear ribonucleoproteinDisease genesSm classGemins 2ProteinSnRNANovel assayModular compositionMultiple interactionsComplexesGemin5Major component