2015
Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization
Neuenkirchen N, Englbrecht C, Ohmer J, Ziegenhals T, Chari A, Fischer U. Reconstitution of the human U snRNP assembly machinery reveals stepwise Sm protein organization. The EMBO Journal 2015, 34: 1925-1941. PMID: 26069323, PMCID: PMC4547896, DOI: 10.15252/embj.201490350.Peer-Reviewed Original ResearchConceptsSMN complexAssembly machinerySm proteinsSpliceosomal U snRNPsDetailed molecular dissectionTrans-acting factorsSpontaneous conformational changesBiochemical reconstitutionPRMT5 complexU snRNPsProtein complexesMolecular dissectionProtein substratesProtein organizationIndividual proteinsConformational changesFaithful deliveryMode of actionRecombinant sourcesCoordinated actionBrownian machineMechanistic insightsSnRNPsProteinAssembly reaction
2008
Deciphering the assembly pathway of Sm‐class U snRNPs
Neuenkirchen N, Chari A, Fischer U. Deciphering the assembly pathway of Sm‐class U snRNPs. FEBS Letters 2008, 582: 1997-2003. PMID: 18348870, DOI: 10.1016/j.febslet.2008.03.009.Peer-Reviewed Original ResearchConceptsU snRNPsAssembly pathwayUridine-rich small nuclear ribonucleoproteinsProtein arginine methyltransferase 5SMN complex functionTrans-acting factorsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinCommon proteinsSurvival motor neuronSm classSnRNPsProteinPathwayMotor neuronsRibonucleoproteinRecent progressRNASMNAssemblyLarge numberVitroVivoComplexes