1998
How E. coli DNA polymerase I (klenow fragment) distinguishes between deoxy- and dideoxynucleotides11Edited by A. R Fersht
Astatke M, Grindley N, Joyce C. How E. coli DNA polymerase I (klenow fragment) distinguishes between deoxy- and dideoxynucleotides11Edited by A. R Fersht. Journal Of Molecular Biology 1998, 278: 147-165. PMID: 9571040, DOI: 10.1006/jmbi.1998.1672.Peer-Reviewed Original ResearchConceptsMutant derivativesWild-type Klenow fragmentKlenow fragmentTernary complexAmino acid residuesE. coli DNA polymerase IIncorporation of dNTPsDNA polymerase IDNTP ternary complexPolymerase IAcid residuesPhosphoryl transferState kinetic parametersConformational changesNatural substratePositions 762DNA polymeraseEnzyme DNAKlenow fragment DNA polymeraseDNTPsIncoming dNTPDNTPSide chain resultsRibose moietyDideoxynucleotides
1997
Site-specific recombination: Synapsis and strand exchange revealed
Grindley N. Site-specific recombination: Synapsis and strand exchange revealed. Current Biology 1997, 7: r608-r612. PMID: 9368738, DOI: 10.1016/s0960-9822(06)00314-9.Peer-Reviewed Original ResearchBinding SitesDNA NucleotidyltransferasesIntegrasesRecombinasesRecombination, GeneticStructure-Activity RelationshipBiochemical and mutational studies of the 5′-3′ exonuclease of DNA polymerase I of Escherichia coli11Edited by A. R. Fersht
Xu Y, Derbyshire V, Ng K, Sun X, Grindley N, Joyce C. Biochemical and mutational studies of the 5′-3′ exonuclease of DNA polymerase I of Escherichia coli11Edited by A. R. Fersht. Journal Of Molecular Biology 1997, 268: 284-302. PMID: 9159471, DOI: 10.1006/jmbi.1997.0967.Peer-Reviewed Original Research
1995
Catalytic residues of gamma delta resolvase act in cis.
Boocock M, Zhu X, Grindley N. Catalytic residues of gamma delta resolvase act in cis. The EMBO Journal 1995, 14: 5129-5140. PMID: 7588641, PMCID: PMC394616, DOI: 10.1002/j.1460-2075.1995.tb00195.x.Peer-Reviewed Original ResearchBase SequenceBinding SitesCrossing Over, GeneticDNA NucleotidyltransferasesDNA Topoisomerases, Type IDNA Transposable ElementsGenetic Complementation TestModels, GeneticModels, MolecularMolecular Sequence DataPlasmidsRecombination, GeneticStructure-Activity RelationshipSubstrate SpecificityTransposases
1993
Protein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis.
Hughes R, Rice P, Steitz T, Grindley N. Protein‐protein interactions directing resolvase site‐specific recombination: a structure‐function analysis. The EMBO Journal 1993, 12: 1447-1458. PMID: 8385604, PMCID: PMC413356, DOI: 10.1002/j.1460-2075.1993.tb05788.x.Peer-Reviewed Original Research
1986
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.
Hatfull G, Grindley N. Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 5429-5433. PMID: 3016704, PMCID: PMC386300, DOI: 10.1073/pnas.83.15.5429.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesCysteineDNA Transposable ElementsDNA-Binding ProteinsDNA, BacterialMethylationNucleotidyltransferasesRecombination, GeneticSerineStructure-Activity RelationshipTransposasesConceptsSerine 10Resolvase mutantsRecombinational activitySite-specific recombination proteinsProtein-DNA complexesPosition 10Site-specific recombinationRecombination proteinsActive site serineMutant proteinsRecombinational crossover pointMutantsResolvaseRecombinational sitesLeucine changeSerineSite ICysteine changeGel electrophoresisSpecific defectsComplex formationCointegrate moleculeTransposon gamma deltaProteinDNA
1984
Mutants of the γδ resolvase: A genetic analysis of the recombination function
Newman B, Grindley N. Mutants of the γδ resolvase: A genetic analysis of the recombination function. Cell 1984, 38: 463-469. PMID: 6088082, DOI: 10.1016/0092-8674(84)90501-4.Peer-Reviewed Original ResearchConceptsAmino acid amino-terminal domainAmino-terminal domainDifferent amino acid substitutionsSite-specific recombinationGamma delta transposonAmino acid substitutionsRecombination functionsTransposon genesIndependent mutantsCatalytic domainRelated recombinasesGenetic analysisDistinct residuesΓδ resolvaseMutantsAcid substitutionsResolvaseRegulatory propertiesCrossover sitesAmino acidsResolvase proteinCointegrate resolutionRecombinasesTransposonGenes