2023
Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon
Micali N, Ma S, Li M, Kim S, Mato-Blanco X, Sindhu S, Arellano J, Gao T, Shibata M, Gobeske K, Duque A, Santpere G, Sestan N, Rakic P. Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon. Science 2023, 382: eadf3786. PMID: 37824652, PMCID: PMC10705812, DOI: 10.1126/science.adf3786.Peer-Reviewed Original Research
2020
Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
Micali N, Kim SK, Diaz-Bustamante M, Stein-O’Brien G, Seo S, Shin JH, Rash BG, Ma S, Wang Y, Olivares NA, Arellano JI, Maynard KR, Fertig EJ, Cross AJ, Bürli RW, Brandon NJ, Weinberger DR, Chenoweth JG, Hoeppner DJ, Sestan N, Rakic P, Colantuoni C, McKay RD. Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates. Cell Reports 2020, 31: 107599. PMID: 32375049, PMCID: PMC7357345, DOI: 10.1016/j.celrep.2020.107599.Peer-Reviewed Original ResearchConceptsNeural stem cellsNeuronal fateProliferative neural stem cellsStem cellsPluripotent linesTelencephalic fateRNA sequencingLineage tracingHuman neural stem cellsGlutamatergic excitatory neuronsMonkey brain sectionsNeuronal trajectoriesCell imagingCortical excitatoryCerebral cortexFateExcitatory neuronsBrain sectionsHuman telencephalonNeuropsychiatric disordersAcute transitionPluripotencyCellsCortexSequencingDissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs
Burke EE, Chenoweth JG, Shin JH, Collado-Torres L, Kim SK, Micali N, Wang Y, Colantuoni C, Straub RE, Hoeppner DJ, Chen HY, Sellers A, Shibbani K, Hamersky GR, Diaz Bustamante M, Phan BN, Ulrich WS, Valencia C, Jaishankar A, Price AJ, Rajpurohit A, Semick SA, Bürli RW, Barrow JC, Hiler DJ, Page SC, Martinowich K, Hyde TM, Kleinman JE, Berman KF, Apud JA, Cross AJ, Brandon NJ, Weinberger DR, Maher BJ, McKay RDG, Jaffe AE. Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs. Nature Communications 2020, 11: 462. PMID: 31974374, PMCID: PMC6978526, DOI: 10.1038/s41467-019-14266-z.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsNeural precursor cellsExpression dataSingle-cell expression dataNeuronal differentiationSequencing read alignmentsInduced pluripotent stem cellsEarly neuronal differentiationPluripotent stem cellsTranscriptomic resourcesIPSC donorNeuronal culturesSubclonal linesNeural differentiationTranscriptomic signaturesHuman neural precursor cellsNeuronal cellsStem cellsPrecursor cellsCell sortingGlobal patternsPowerful modelSubset of neuronsRead alignmentDifferentiation
2014
FGF2 and Insulin Signaling Converge to Regulate Cyclin D Expression in Multipotent Neural Stem Cells
Adepoju A, Micali N, Ogawa K, Hoeppner DJ, McKay RD. FGF2 and Insulin Signaling Converge to Regulate Cyclin D Expression in Multipotent Neural Stem Cells. Stem Cells 2014, 32: 770-778. PMID: 24155149, DOI: 10.1002/stem.1575.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationCyclin DDNAFemaleFibroblast Growth Factor 2InsulinIntracellular SpaceMiceMice, Inbred C57BLModels, BiologicalMultipotent Stem CellsNeural Stem CellsProtein BiosynthesisProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junSignal TransductionTranscription, GeneticConceptsNeural stem cellsMultipotent neural stem cellsStem cellsPost-transcriptional levelPI3K/Akt pathwayTyrosine kinase receptorsPhosphorylation of ERK1/2Cell statesInsulin signalProliferation controlCell lineagesFibroblast growth factorBasic fibroblast growth factorCyclin D expressionMultipotent cellsC-JunD mRNA levelsKinase receptorsNeural precursorsAkt pathwayCell expansionMultipotent natureCentral nervous systemDisease mechanismsC-fos