2016
Direct interaction of the resistance to inhibitors of cholinesterase type 3 protein with the serotonin receptor type 3A intracellular domain
Nishtala SN, Mnatsakanyan N, Pandhare A, Leung C, Jansen M. Direct interaction of the resistance to inhibitors of cholinesterase type 3 protein with the serotonin receptor type 3A intracellular domain. Journal Of Neurochemistry 2016, 137: 528-538. PMID: 26875553, PMCID: PMC4860158, DOI: 10.1111/jnc.13578.Peer-Reviewed Original ResearchConceptsGloeobacter violaceus ligand-gated ion channelPentameric ligand-gated ion channelsLigand-gated ion channelsRIC-3Intracellular domainIon channelsType 3 proteinChaperone protein RIC-3Non-excitable cellsWild-type channelsTransmembrane domainProtein factorsHomologous subunitsPentameric assemblyHeteromeric pentamersChimera consistingInteraction surfaceProteinDirect interactionLaevis oocytesProtein expressionFirst experimental evidenceSpecific interactionsSurface expressionFunctional maturation
2015
Functional Chimeras of GLIC Obtained by Adding the Intracellular Domain of Anion- and Cation-Conducting Cys-Loop Receptors
Mnatsakanyan N, Nishtala SN, Pandhare A, Fiori MC, Goyal R, Pauwels JE, Navetta AF, Ahrorov A, Jansen M. Functional Chimeras of GLIC Obtained by Adding the Intracellular Domain of Anion- and Cation-Conducting Cys-Loop Receptors. Biochemistry 2015, 54: 2670-2682. PMID: 25861708, PMCID: PMC4414916, DOI: 10.1021/acs.biochem.5b00203.Peer-Reviewed Original ResearchConceptsPentameric ligand-gated ion channelsEukaryotic pentameric ligand-gated ion channelsProkaryotic pentameric ligand-gated ion channelsIntracellular domainCys-loop receptorsComplete intracellular domainLarge intracellular domainLigand-gated ion channelsAcetylcholine-binding proteinLarge-scale expressionChaperone protein RIC-3Eukaryotic membersStructural studiesTorpedo nicotinic acetylcholine receptorSequence conservationFunctional chimerasTransmembrane domainRIC-3Eukaryotic channelsSuperfamily membersPentameric assemblyDiverse rolesProton-gated channelsOverall structural featuresNicotinic acetylcholine receptors