2010
High Endothelial Venule Reporter Mice to Probe Regulation of Lymph Node Vasculature
Bentley KL, Stranford S, Liao S, Mounzer RM, Ruddle FH, Ruddle NH. High Endothelial Venule Reporter Mice to Probe Regulation of Lymph Node Vasculature. Advances In Experimental Medicine And Biology 2010, 691: 35-44. PMID: 21153307, DOI: 10.1007/978-1-4419-6612-4_4.Peer-Reviewed Original Research
2007
Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules
Liao S, Bentley K, Lebrun M, Lesslauer W, Ruddle FH, Ruddle NH. Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 4577-4582. PMID: 17360566, PMCID: PMC1838643, DOI: 10.1073/pnas.0700334104.Peer-Reviewed Original ResearchConceptsDNA fragmentsTertiary lymphoid organsExpression of reporterEndogenous gene expressionBAC DNA fragmentsTissue-specific expressionBeta-galactosidase reporter geneHomologous recombination techniquesLymphoid organsLymphoid tissueEffector genesBAC clonesEndogenous genesRegulatory sequencesNasal-associated lymphoid tissueReporter geneGene expressionLacZ constructLTbetaR-Ig treatmentExon IIHEV-like vesselsGenesHigh endothelial venulesMolecular natureRecombination techniques
2004
Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates Relationship to the MECA-79 Epitope
Bistrup A, Tsay D, Shenoy P, Singer MS, Bangia N, Luther SA, Cyster JG, Ruddle NH, Rosen SD. Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates Relationship to the MECA-79 Epitope. American Journal Of Pathology 2004, 164: 1635-1644. PMID: 15111310, PMCID: PMC1615668, DOI: 10.1016/s0002-9440(10)63722-4.Peer-Reviewed Original ResearchConceptsHigh endothelial venulesMECA-79 epitopeLymph nodesMECA-79Endothelial venulesHEC-GlcNAc6STHigh Endothelial Venule-Like VesselsLuminal stainingL-selectinMECA-79 monoclonal antibodyNull miceNonobese diabetic (NOD) miceHEV-like vesselsLymph nodes resultsHigh endothelial cellsLymphoid neogenesisDiabetic miceLymphocyte rollingLymphocyte homingMonoclonal antibodiesEndothelial cellsMiceConcomitant expressionEpitopesVenules
2003
Ectopic LTαβ Directs Lymphoid Organ Neogenesis with Concomitant Expression of Peripheral Node Addressin and a HEV-restricted Sulfotransferase
Drayton DL, Ying X, Lee J, Lesslauer W, Ruddle NH. Ectopic LTαβ Directs Lymphoid Organ Neogenesis with Concomitant Expression of Peripheral Node Addressin and a HEV-restricted Sulfotransferase. Journal Of Experimental Medicine 2003, 197: 1153-1163. PMID: 12732657, PMCID: PMC2193975, DOI: 10.1084/jem.20021761.Peer-Reviewed Original ResearchConceptsHigh endothelial venulesPeripheral node addressinLymphoid organogenesisLT-alphaB cell compartmentalizationMucosal addressin cell adhesion moleculeAlpha betaLymph node functionB-cell areasAntigen presenting cellsLymphoid neogenesisPancreatic infiltratesPNAd expressionLymphoid chemokinesFDC networksMononuclear infiltrateAlpha micePresenting cellsEndothelial venulesCell adhesion moleculeCell accumulationLT-betaAdhesion moleculesNode functionPancreata
2001
Sulfation of L-Selectin Ligands by an HEV-Restricted Sulfotransferase Regulates Lymphocyte Homing to Lymph Nodes
Hemmerich S, Bistrup A, Singer M, van Zante A, Lee J, Tsay D, Peters M, Carminati J, Brennan T, Carver-Moore K, Leviten M, Fuentes M, Ruddle N, Rosen S. Sulfation of L-Selectin Ligands by an HEV-Restricted Sulfotransferase Regulates Lymphocyte Homing to Lymph Nodes. Immunity 2001, 15: 237-247. PMID: 11520459, DOI: 10.1016/s1074-7613(01)00188-1.Peer-Reviewed Original ResearchConceptsHigh endothelial venulesLymph nodesL-selectinLuminal aspectChronic inflammatory sitesHEC-GlcNAc6STImportant therapeutic targetL-selectin ligandsMECA-79Lymphocyte traffickingEndothelial venulesInflammatory sitesTherapeutic targetLymphocyte homingLymphocyte bindingGenetic deletionSpecific ligandsLigand activityRecombinant L-selectinSulfotransferaseCritical roleEssential posttranslational modificationVenules