FACS-Assisted Microarray Profiling Implicates Novel Genes and Pathways in Zebrafish Gastrointestinal Tract Development
Stuckenholz C, Lu L, Thakur P, Kaminski N, Bahary N. FACS-Assisted Microarray Profiling Implicates Novel Genes and Pathways in Zebrafish Gastrointestinal Tract Development. Gastroenterology 2009, 137: 1321-1332. PMID: 19563808, PMCID: PMC2785077, DOI: 10.1053/j.gastro.2009.06.050.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell SeparationChromosomes, Human, Pair 8Digestive System NeoplasmsFlow CytometryGastrointestinal TractGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Expression Regulation, NeoplasticGene Regulatory NetworksGreen Fluorescent ProteinsHumansIn Situ HybridizationLarvaMicroRNAsOligonucleotide Array Sequence AnalysisOrganogenesisReproducibility of ResultsTime FactorsZebrafishZebrafish ProteinsConceptsFluorescence-activated cell sortingNovel genesGreen fluorescent proteinGene networksPutative transcription factorTransgenic zebrafish lineZebrafish Danio rerioPhosphatidylinositol-3-kinase (PI3K) pathwayExcellent model systemDevelopmental time pointsChromosome arm 8qGastrointestinal developmentZebrafish lineHuman orthologDanio rerioTranscription factorsKinase pathwayMicroarray profilingFluorescent proteinGFP expressionGenesNovel pathwayGFP cellsCell sortingOrganogenesis