2022
Readily Available Tissue-Engineered Vascular Grafts Derived From Human Induced Pluripotent Stem Cells
Luo J, Qin L, Park J, Kural MH, Huang Y, Shi X, Riaz M, Wang J, Ellis MW, Anderson CW, Yuan Y, Ren Y, Yoder MC, Tellides G, Niklason LE, Qyang Y. Readily Available Tissue-Engineered Vascular Grafts Derived From Human Induced Pluripotent Stem Cells. Circulation Research 2022, 130: 925-927. PMID: 35189711, PMCID: PMC9113663, DOI: 10.1161/circresaha.121.320315.Peer-Reviewed Original Research
2021
Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationHumansInduced Pluripotent Stem CellsMuscle, Smooth, VascularMyocytes, Smooth MuscleTissue EngineeringConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2020
Efficient Differentiation of Human Induced Pluripotent Stem Cells into Endothelial Cells under Xenogeneic-free Conditions for Vascular Tissue Engineering
Luo J, Shi X, Lin Y, Yuan Y, Kural MH, Wang J, Ellis MW, Anderson CW, Zhang SM, Riaz M, Niklason LE, Qyang Y. Efficient Differentiation of Human Induced Pluripotent Stem Cells into Endothelial Cells under Xenogeneic-free Conditions for Vascular Tissue Engineering. Acta Biomaterialia 2020, 119: 184-196. PMID: 33166710, PMCID: PMC8133308, DOI: 10.1016/j.actbio.2020.11.007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Vessel ProsthesisCell DifferentiationEndothelial CellsHumansInduced Pluripotent Stem CellsTissue EngineeringConceptsVascular tissue engineeringTissue engineeringSmall-diameter TEVGsDynamic bioreactor systemShear stressBioreactor systemCell alignmentVascular graftsXenogeneic-free conditionsEngineeringEndothelializationTEVGsApplicationsEndothelial cellsConditionsHuman induced pluripotent stem cellsAnimal-derived reagentsXenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHumansInduced Pluripotent Stem CellsMiceMuscle, Smooth, VascularMyocytes, Smooth MuscleTissue EngineeringConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffolds
2019
Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes
Park J, Anderson CW, Sewanan LR, Kural MH, Huang Y, Luo J, Gui L, Riaz M, Lopez CA, Ng R, Das SK, Wang J, Niklason L, Campbell SG, Qyang Y. Modular design of a tissue engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes. Acta Biomaterialia 2019, 102: 220-230. PMID: 31634626, PMCID: PMC7227659, DOI: 10.1016/j.actbio.2019.10.019.Peer-Reviewed Original ResearchConceptsSingle-ventricle cardiac defectsHuman umbilical arteryPluripotent stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesFontan procedureSurgical interventionSVD patientsCell-derived cardiomyocytesFontan conduitTherapeutic potentialEngineered Heart TissueVascular conduitsCongenital disorderDesign strategyVariety of complicationsEfficient electrical conductivitySingle ventricle heartPrimary cardiac fibroblastsFontan circulationHeart failureCorrective surgeryUmbilical arteryPulmonary circulationBiodegradable materialsPulmonary system