2022
Rainfall and other meteorological factors as drivers of urban transmission of leptospirosis
Cunha M, Costa F, Ribeiro GS, Carvalho MS, Reis RB, Nery N, Pischel L, Gouveia EL, Santos AC, Queiroz A, Wunder EA, Reis MG, Diggle PJ, Ko AI. Rainfall and other meteorological factors as drivers of urban transmission of leptospirosis. PLOS Neglected Tropical Diseases 2022, 16: e0007507. PMID: 35404948, PMCID: PMC9022820, DOI: 10.1371/journal.pntd.0007507.Peer-Reviewed Original Research
2020
Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources
Casanovas-Massana A, Hamond C, Santos LA, de Oliveira D, Hacker KP, Balassiano I, Costa F, Medeiros MA, Reis MG, Ko AI, Wunder EA. Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources. International Journal Of Systematic And Evolutionary Microbiology 2020, 70: 1450-1456. PMID: 31184568, PMCID: PMC10197099, DOI: 10.1099/ijsem.0.003480.Peer-Reviewed Original Research
2016
Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus)
DE OLIVEIRA D, FIGUEIRA CP, ZHAN L, PERTILE AC, PEDRA GG, GUSMÃO IM, WUNDER EA, RODRIGUES G, RAMOS EA, KO AI, CHILDS JE, REIS MG, COSTA F. Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus). Epidemiology And Infection 2016, 144: 2420-2429. PMID: 27019024, PMCID: PMC5437553, DOI: 10.1017/s0950268816000637.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionBreast tissuePresence of leptospiresNorway ratsMajor public health concernMilk-borne transmissionMajor pathological changesReal-time polymerase chain reactionPublic health concernQuantitative real-time polymerase chain reactionPathogenic Leptospira sppBreast tissue samplesChronic carriagePathological changesLactating ratsRatsHealth concernImmunofluorescence assaysLeptospira sppGenus LeptospiraTissue samplesUrban slumsChain reactionLeptospiresLeptospira
2001
Evaluation of Recombinant Leptospira Antigen-Based Enzyme-Linked Immunosorbent Assays for the Serodiagnosis of Leptospirosis
Flannery B, Costa D, Carvalho F, Guerreiro H, Matsunaga J, Da Silva E, Ferreira A, Riley L, Reis M, Haake D, Ko A. Evaluation of Recombinant Leptospira Antigen-Based Enzyme-Linked Immunosorbent Assays for the Serodiagnosis of Leptospirosis. Journal Of Clinical Microbiology 2001, 39: 3303-3310. PMID: 11526167, PMCID: PMC88335, DOI: 10.1128/jcm.39.9.3303-3310.2001.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayIgG enzyme-linked immunosorbent assaySerodiagnosis of leptospirosisHealthy individualsImmunosorbent assayTest-positive individualsCases of leptospirosisHigh-incidence regionsLyme disease patientsMicroscopic agglutination testConvalescent phaseHepatitis patientsAcute phaseDengue patientsDisease patientsPatient seraHealthy residentsLeptospira antigensCutoff valueDiagnostic utilityPatientsAgglutination testUseful antigenLeptospirosisAntigenLeptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans
Guerreiro H, Croda J, Flannery B, Mazel M, Matsunaga J, Reis M, Levett P, Ko A, Haake D. Leptospiral Proteins Recognized during the Humoral Immune Response to Leptospirosis in Humans. Infection And Immunity 2001, 69: 4958-4968. PMID: 11447174, PMCID: PMC98588, DOI: 10.1128/iai.69.8.4958-4968.2001.Peer-Reviewed Original ResearchConceptsHumoral immune responseImmune responseNatural infectionLeptospiral proteinsInfection-associated antigenConvalescent-phase seraPositive reactionConvalescent phaseHumoral responseHealthy individualsHuman leptospirosisControl individualsVaccine designImmunodominant antigensQuantitative immunoblot analysisTwo-dimensional immunoblotsProtein antigensPathogenic leptospiral strainsGenus LeptospiraInfectionAntigenLeptospirosisSerumImmunodominant proteinsPatientsIdentification of New Repetitive Element inLeptospira interrogans Serovar Copenhageni and Its Application to PCR-Based Differentiation ofLeptospira Serogroups
Barocchi M, Ko A, Ferrer S, Faria M, Reis M, Riley L. Identification of New Repetitive Element inLeptospira interrogans Serovar Copenhageni and Its Application to PCR-Based Differentiation ofLeptospira Serogroups. Journal Of Clinical Microbiology 2001, 39: 191-195. PMID: 11136769, PMCID: PMC87700, DOI: 10.1128/jcm.39.1.191-195.2001.Peer-Reviewed Original Research