2018
Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells
Lu M, Li JR, Alvarez-Lugo L, Li Y, Yu S, Li X, Shi B, Chai TC. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. American Journal Of Physiology - Cell Physiology 2018, 314: c643-c653. PMID: 29466671, DOI: 10.1152/ajpcell.00339.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic AMP-Dependent Protein KinasesFemaleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLipopolysaccharide ReceptorsLipopolysaccharidesLymphocyte Antigen 96Membrane PotentialsMice, Inbred C57BLPatch-Clamp TechniquesPotassiumSignal TransductionToll-Like Receptor 4Urinary BladderUrinary Tract InfectionsUrotheliumConceptsBK channel activityBK channelsChannel activityUmbrella cellsUrinary tract infectionFemale C57BL6 micePotassium channel inhibitorsUropathogenic Escherichia coliProtein kinase ATract infectionsLPS effectsC57BL6 miceBladder urotheliumLamina propriaMin of exposureChannel inhibitorsBacterial infectionsLPS receptorUrothelial tissuesUrothelial cellsUrotheliumLipopolysaccharidePotassium channelsRT-PCRPKA blocker
2016
Granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) is released by female mouse bladder urothelial cells and expressed by the urothelium as an early response to lipopolysaccharides (LPS)
Li Y, Lu M, Alvarez‐Lugo L, Chen G, Chai TC. Granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) is released by female mouse bladder urothelial cells and expressed by the urothelium as an early response to lipopolysaccharides (LPS). Neurourology And Urodynamics 2016, 36: 1020-1025. PMID: 27337494, DOI: 10.1002/nau.23057.Peer-Reviewed Original ResearchConceptsBladder urotheliumCyclooxygenase-2GM-CSFCyclooxygenase-1GM-CSFRαBladder tissueMRNA expressionTumor necrosis factor αCOX-2 mRNA expressionVivo LPS exposureVascular endothelial growth factorNecrosis factor αDose-dependent releaseIncrease of VEGFGranulocyte-macrophage colony-stimulating factor (GM-CSF) signalingBladder urothelial cellsConcentrations of lipopolysaccharideGM-CSF mRNA expressionEndothelial growth factorGM-CSF antibodyMouse bladder urotheliumGM-CSF activityLPS exposurePain transductionSingle dose