1999
Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells
Shi C, Ryan J, French A, Coca‐Prados M, Kelly M. Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells. The Journal Of Physiology 1999, 521: 57-67. PMID: 10562334, PMCID: PMC2269649, DOI: 10.1111/j.1469-7793.1999.00057.x.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsCalciumCell LineChloride ChannelsCiliary BodyEnzyme InhibitorsEpithelial CellsNiflumic AcidOsmotic PressurePatch-Clamp TechniquesPhosphorylationProtein Kinase InhibitorsRabbitsSecond Messenger SystemsStilbenesConceptsNon-pigmented ciliary epithelial cellsWhole-cell currentsCiliary epithelial cellsWhole-cell patch-clamp recording techniquePatch-clamp recording techniquesEpithelial cellsTime courseSlower time courseDisulfonic acidSmall conductanceWhole-cell conductanceCl- current amplitudeExposure of cellsVoltage-sensitive blockCl- currentVoltage-independent blockNiflumic acidRecording techniquesDepolarized potentialsCurrent activationAmplitude of currentsOutward rectificationCell swellingNPCE cellsHyposmotic shock
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1997
Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1997, 273: c1354-c1361. PMID: 9357781, DOI: 10.1152/ajpcell.1997.273.4.c1354.Peer-Reviewed Original ResearchConceptsCiliary epithelial cellsNonpigmented ciliary epithelial cellsWhole-cell currentsEpithelial cellsAdenosine receptorsNPE cellsA2 adenosine receptorsMultiple purinergic receptorsCl- channel blockerHuman NPE cellsIntraocular pressureAdenosine actionPurinergic receptorsHCE cellsRabbit ciliary epitheliumCiliary epitheliumNiflumic acidBovine cellsReceptors