2008
Heterozygous expression of myocilin glaucoma mutants increases secretion of the mutant forms and reduces extracellular processed myocilin.
Aroca-Aguilar JD, Sánchez-Sánchez F, Martínez-Redondo F, Coca-Prados M, Escribano J. Heterozygous expression of myocilin glaucoma mutants increases secretion of the mutant forms and reduces extracellular processed myocilin. Molecular Vision 2008, 14: 2097-108. PMID: 19023451, PMCID: PMC2585175.Peer-Reviewed Original ResearchConceptsWild-type myocilinWild-type proteinMyocilin mutantsMutant myocilinMutant formsProteolytic processingMissense mutant formsHEK 293T cellsMyocilin geneMutant proteinsSecretory pathwayUnidentified functionExtracellular proteinsMutantsEndoproteolytic processingRecombinant mutantsMyocilin secretionCellular fractionsHeterozygous expressionMyocilinProteinUnknown mechanismExtracellular amountSDS-PAGEHeterozygous state
2000
Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells
Counillon L, Touret N, Bidet M, Peterson-Yantorno K, Coca-Prados M, Stuart-Tilley A, Wilhelm S, Alper S, Civan M. Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells. Pflügers Archiv - European Journal Of Physiology 2000, 440: 667-678. PMID: 11007305, DOI: 10.1007/s004240000302.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAntiportersCattleCell Line, TransformedChloride-Bicarbonate AntiportersCiliary BodyEpithelial CellsImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceMicroscopy, VideoModels, BiologicalPigmentationReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSLC4A ProteinsSodiumSodium-Hydrogen ExchangersConceptsAqueous humor formationCl-/HCO3Epithelial cell layerTranscriptase-polymerase chain reaction amplificationPE cellsCell layerHuman ciliary bodyAE2 Cl-/HCO3Medical therapyNHE-1 isoformCiliary epithelial cellsCiliary bodyAqueous humorCiliary epithelial bilayerPolymerase chain reaction amplificationChain reaction amplificationNHE-1Rabbit ciliary epitheliumCiliary epitheliumEpithelial cellsBovine pigmented ciliary epithelial cellsGap junctionsRegulatory volume increasePigmented ciliary epithelial cellsExternal Cl
1997
Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium
Ortego J, Coca‐Prados M. Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1997, 69: 1829-1839. PMID: 9349525, DOI: 10.1046/j.1471-4159.1997.69051829.x.Peer-Reviewed Original ResearchMeSH KeywordsAspartic Acid EndopeptidasesCell LineCiliary BodyColforsinDexamethasoneDNA PrimersEnzyme InductionEpithelial CellsFurinGene Expression RegulationHumansIsoproterenolModels, BiologicalNerve Growth FactorsNeurosecretory SystemsNeurotensinPolymerase Chain ReactionProprotein Convertase 2Proprotein ConvertasesReceptors, NeurotensinSubtilisinsTetradecanoylphorbol AcetateTimololTranscriptional ActivationUp-RegulationConceptsOcular ciliary epitheliumDifferential gene inductionImportant physiological functionsHuman ocular ciliary epitheliumHuman cell linesTransduction pathwaysGene inductionGene expressionCiliary epitheliumBeta2-adrenergic receptorMolecular characterizationPhysiological functionsCell extractsODM-2Human ciliary epitheliumNeuroepithelial cellsSame inducersPhorbol esterCell linesNeuroendocrine tissuesExpressionGrowth factorParacrine mechanismsPC2 expressionNerve growth factor
1991
Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion
Ghosh S, Hernando N, Martín‐Alonso J, Martin‐Vasallo P, Coca‐Prados M. Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion. Journal Of Cellular Physiology 1991, 149: 184-194. PMID: 1660898, DOI: 10.1002/jcp.1041490203.Peer-Reviewed Original Research
1982
Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion
Coca-Prados M, Yu H, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion. Journal Of Virology 1982, 44: 603-609. PMID: 6292520, PMCID: PMC256304, DOI: 10.1128/jvi.44.2.603-609.1982.Peer-Reviewed Original ResearchConceptsSimian virus 40 nucleoprotein complexesNucleoprotein complexesSV40 chromatinNuclease digestionDNA fragmentsCapsid shellMature virionsNucleosome repeat lengthMicrococcal nuclease digestionGenome-sized DNASV40 nucleoprotein complexesIntracellular formNovel DNA fragmentsStructure of DNAAssembly intermediatesNucleosomal DNA fragmentsMicrococcal nucleaseVirion assemblyMild digestionSV40 virionsChromatinFraction of intermediatesVirion assembly intermediatesVirion maturationCapsid protein