2002
Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium
Cullinane A, Leung P, Ortego J, Coca-Prados M, Harvey B. Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium. British Journal Of Ophthalmology 2002, 86: 676. PMID: 12034692, PMCID: PMC1771149, DOI: 10.1136/bjo.86.6.676.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICalciumCell SizeCells, CulturedCiliary BodyElectrophysiologyHumansLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPigment Epithelium of EyePotassium Channels, Calcium-ActivatedReceptors, AngiotensinRenin-Angiotensin SystemReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsHNPE cellsAngiotensin IINon-pigmented epitheliumSecretory functionAddition of AIIRenin-angiotensin system componentsRenin-angiotensin system expressionIon channel activityPotassium ion channel activityActive BK channelsExpression of angiotensinogenAqueous humor dynamicsTreatment of glaucomaChannel activitySingle-channel patch-clamp techniquesPatch-clamp techniqueBK channel activationLarge-conductance potassium channelsHuman ciliary bodyPolymerase chain reaction analysisReceptor antagonistCurrent armamentariumCiliary bodyChain reaction analysisRAS expression
1992
Whole cell patch clamping of ciliary epithelial cells during anisosmotic swelling
Yantorno R, Carre D, Coca-Prados M, Krupin T, Civan M. Whole cell patch clamping of ciliary epithelial cells during anisosmotic swelling. American Journal Of Physiology 1992, 262: c501-c509. PMID: 1539636, DOI: 10.1152/ajpcell.1992.262.2.c501.Peer-Reviewed Original Research
1991
Detection and characterization of Ca2+-activated K+ channels in transformed cells of human non-pigmented ciliary epithelium
Barros F, Lòpez-Briones L, Coca-Prados M, Belmonte C. Detection and characterization of Ca2+-activated K+ channels in transformed cells of human non-pigmented ciliary epithelium. Current Eye Research 1991, 10: 731-738. PMID: 1914505, DOI: 10.3109/02713689109013867.Peer-Reviewed Original Research
1989
Effect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells.
Helbig H, Korbmacher C, Wohlfarth J, Coroneo M, Lindschau C, Quass P, Haller H, Coca-Prados M, Wiederholt M. Effect of acetylcholine on membrane potential of cultured human nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1989, 30: 890-6. PMID: 2722445.Peer-Reviewed Original ResearchConceptsAcetylcholine-induced responsesCiliary epithelial cellsCytoplasmic Ca2Epithelial cellsEffects of acetylcholineAddition of acetylcholineMembrane potentialMobilization of Ca2M atropineRole of Ca2M acetylcholineMM quinidineChannel blockersFura-2Sustained depolarizationAcetylcholineIntracellular storesInitial hyperpolarizationTransient hyperpolarizationCell membrane potentialMicroelectrode techniquesCultured humanHyperpolarizationFree mediumOrigin-defective mutant