2014
Quantitative bioimaging of trace elements in the human lens by LA-ICP-MS
Konz I, Fernández B, Fernández ML, Pereiro R, González-Iglesias H, Coca-Prados M, Sanz-Medel A. Quantitative bioimaging of trace elements in the human lens by LA-ICP-MS. Analytical And Bioanalytical Chemistry 2014, 406: 2343-2348. PMID: 24500754, DOI: 10.1007/s00216-014-7617-y.Peer-Reviewed Original ResearchConceptsMatrix-matched laboratory standardsMass spectrometryIsotope dilution mass spectrometryPlasma mass spectrometryDilution mass spectrometryHuman eye lensesAnalysis of FeDepth profiling analysisQuantitative bioimagingThin gold filmMatrix effectsExternal calibrationID-ICPLaser ablation cellQuantification purposesLA-ICPStandard solutionsGold filmCuSpectrometryHomogeneous distributionAblation cellLaser ablationFeMS
2012
The Stoichiometric Transition from Zn6Cu1-Metallothionein to Zn7-Metallothionein Underlies the Up-regulation of Metallothionein (MT) Expression QUANTITATIVE ANALYSIS OF MT-METAL LOAD IN EYE CELLS*
Alvarez L, Gonzalez-Iglesias H, Garcia M, Ghosh S, Sanz-Medel A, Coca-Prados M. The Stoichiometric Transition from Zn6Cu1-Metallothionein to Zn7-Metallothionein Underlies the Up-regulation of Metallothionein (MT) Expression QUANTITATIVE ANALYSIS OF MT-METAL LOAD IN EYE CELLS*. Journal Of Biological Chemistry 2012, 287: 28456-28469. PMID: 22722935, PMCID: PMC3436589, DOI: 10.1074/jbc.m112.365015.Peer-Reviewed Original ResearchConceptsInflammatory eye diseaseRetinal ganglion cellsPresence of TNFαCorneal epithelial cell lineInflammatory cytokinesProinflammatory cytokinesEpithelial cell lineSynthesis of metallothioneinGanglion cellsEye diseaseLevels of metallothioneinCorneal tissueCombination of zincEndothelial cellsCytokinesMT1 isoformsIL1αEffect of zincGene expressionCadaver eyesConcentration of metallothioneinEye cellsMicroarray-based analysisZinc exposureMT isoforms