2008
Expression and purification of functional recombinant human pigment epithelium-derived factor (PEDF) secreted by the yeast Pichia pastoris
Sánchez-Sánchez F, Aroca-Aguilar JD, Segura I, Ramírez-Castillejo C, Riese HH, Coca-Prados M, Escribano J. Expression and purification of functional recombinant human pigment epithelium-derived factor (PEDF) secreted by the yeast Pichia pastoris. Journal Of Biotechnology 2008, 134: 193-201. PMID: 18282627, DOI: 10.1016/j.jbiotec.2008.01.005.Peer-Reviewed Original ResearchConceptsPigment epithelium-derived factorEpithelium-derived factorRecombinant pigment epithelium-derived factorRecombinant human pigment epithelium-derived factorHuman pigment epithelium-derived factorCerebellar granule cell survivalPotential therapeutic agentGranule cell survivalFull-length PEDFStem cell self-renewal propertiesCell-based therapiesTherapeutic roleOcular diseasesTherapeutic agentsSelf-renewal propertiesEndothelial cell migrationLiquid chromatographyCell linesRPEDFCell survivalHigh-performance liquid chromatographyCell migrationYeast Pichia pastorisLow pressure liquid chromatographyPichia pastoris
1982
Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion
Coca-Prados M, Yu H, Hsu M. Intracellular forms of simian virus 40 nucleoprotein complexes. IV. Micrococcal nuclease digestion. Journal Of Virology 1982, 44: 603-609. PMID: 6292520, PMCID: PMC256304, DOI: 10.1128/jvi.44.2.603-609.1982.Peer-Reviewed Original ResearchConceptsSimian virus 40 nucleoprotein complexesNucleoprotein complexesSV40 chromatinNuclease digestionDNA fragmentsCapsid shellMature virionsNucleosome repeat lengthMicrococcal nuclease digestionGenome-sized DNASV40 nucleoprotein complexesIntracellular formNovel DNA fragmentsStructure of DNAAssembly intermediatesNucleosomal DNA fragmentsMicrococcal nucleaseVirion assemblyMild digestionSV40 virionsChromatinFraction of intermediatesVirion assembly intermediatesVirion maturationCapsid protein