2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMPChloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Chloride dependent intracellular pH effects of external ATP in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 443-447. PMID: 12045894, DOI: 10.1076/ceyr.23.6.443.6967.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnti-Bacterial AgentsCalciumCells, CulturedChloride-Bicarbonate AntiportersChloridesCiliary BodyFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationMacrolidesMicroscopy, FluorescencePigment Epithelium of EyeProton-Translocating ATPasesReceptors, PurinergicSpectrometry, FluorescenceSuraminUp-RegulationConceptsNon-pigmented ciliary body epitheliumHNPE cellsCiliary body epitheliumAcetoxymethyl ester formBody epitheliumIntracellular calcium levelsCell-permeable calcium chelatorFluorescent probe BCECFReceptor inhibitorsIntracellular calciumPurinergic receptorsCalcium levelsDiisothiocyanatostilbene-2Extracellular adenosineSustained increaseMM suraminInhibitor amilorideExtracellular ATPCalcium chelatorEster formMicroM ATPATPase inhibitorEpitheliumInhibitorsIntracellularExtracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium
Cullinane A, Coca-Prados M, Harvey B. Extracellular ATP effects on calcium signaling in cultured human non-pigmented ciliary body epithelium. Current Eye Research 2001, 23: 448-454. PMID: 12045895, DOI: 10.1076/ceyr.23.6.448.6964.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCalciumCalcium-Transporting ATPasesCells, CulturedCiliary BodyFluorescent DyesFura-2HumansMicroscopy, FluorescencePigment Epithelium of EyeReceptors, Purinergic P2Receptors, Purinergic P2Y2Signal TransductionSodium-Calcium ExchangerSpectrometry, FluorescenceThapsigarginConceptsNon-pigmented ciliary body epitheliumCiliary body epitheliumHNPE cellsIntracellular storesBody epitheliumFluorescent dye fura-2Extracellular ATP effectP2Y2 receptor activationDye fura-2Calcium signalsATPase pumpPresence of thapsigarginCapacitative calcium entrySodium-free solutionIntracellular calciumPurinergic receptorsFura-2Therapeutic targetCalcium entryPotency orderReceptor activationSustained increaseL-typeExtracellular ATPOscillatory increases
2000
Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells
Mitchell C, Peterson‐Yantorno K, Coca‐Prados M, Civan M. Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells. The Journal Of Physiology 2000, 525: 183-193. PMID: 10811736, PMCID: PMC2269939, DOI: 10.1111/j.1469-7793.2000.00183.x.Peer-Reviewed Original Research