2020
CPAMD8 loss-of-function underlies non-dominant congenital glaucoma with variable anterior segment dysgenesis and abnormal extracellular matrix
Bonet-Fernández J, Aroca-Aguilar J, Corton M, Ramírez A, Alexandre-Moreno S, García-Antón M, Salazar J, Ferre-Fernández J, Atienzar-Aroca R, Villaverde C, Iancu I, Tamayo A, Méndez-Hernández C, Morales-Fernández L, Rojas B, Ayuso C, Coca-Prados M, Martinez-de-la-Casa J, García-Feijoo J, Escribano J. CPAMD8 loss-of-function underlies non-dominant congenital glaucoma with variable anterior segment dysgenesis and abnormal extracellular matrix. Human Genetics 2020, 139: 1209-1231. PMID: 32274568, DOI: 10.1007/s00439-020-02164-0.Peer-Reviewed Original ResearchMeSH KeywordsAdultAlpha-MacroglobulinsAnimalsAnterior ChamberCase-Control StudiesComplement C3CRISPR-Cas SystemsEmbryo, NonmammalianExtracellular MatrixEye AbnormalitiesFemaleGene EditingGene ExpressionGenes, RecessiveGlaucomaHigh-Throughput Nucleotide SequencingHumansLoss of Function MutationMaleMiddle AgedPedigreeTrabecular MeshworkTrabeculectomyTrypsin Inhibitor, Kazal PancreaticZebrafishConceptsZebrafish embryosAnterior segment dysgenesisExtracellular matrixPrimary congenital glaucomaNext-generation DNA sequencingGross developmental abnormalitiesFunction pathogenic mechanismQuantitative reverse transcription PCRAbnormal extracellular matrixCongenital glaucomaCRISPR/Mesenchyme-like cellsTrabecular meshwork cellsReverse transcription-PCRUnknown functionExtracellular matrix disorganizationDNA sequencingGenesGenetic alterationsEmbryosMeshwork cellsDevelopmental abnormalitiesTranscription-PCRAnterior chamber angleDisease Role
2009
Functional Role of Proteolytic Processing of Recombinant Myocilin in Self-Aggregation
Aroca-Aguilar JD, Martínez-Redondo F, Sánchez-Sánchez F, Coca-Prados M, Escribano J. Functional Role of Proteolytic Processing of Recombinant Myocilin in Self-Aggregation. Investigative Ophthalmology & Visual Science 2009, 51: 72-78. PMID: 19696176, PMCID: PMC2869055, DOI: 10.1167/iovs.09-4118.Peer-Reviewed Original Research
2007
New perspectives in aqueous humor secretion and in glaucoma: The ciliary body as a multifunctional neuroendocrine gland
Coca-Prados M, Escribano J. New perspectives in aqueous humor secretion and in glaucoma: The ciliary body as a multifunctional neuroendocrine gland. Progress In Retinal And Eye Research 2007, 26: 239-262. PMID: 17321191, DOI: 10.1016/j.preteyeres.2007.01.002.Peer-Reviewed Original ResearchConceptsIntraocular pressureCiliary bodyAqueous humor secretionAqueous humorCiliary epitheliumGlaucoma-associated genesImmune privilege statusDevelopment of glaucomaGlutamate-metabolizing enzymesPathophysiology of glaucomaAnti-angiogenic factorsSteroid-converting enzymesDiurnal circadian rhythmCiliary blood flowOcular ciliary bodyAnterior segmentBlood flowMultifactorial eventGlaucomaNeuroendocrine systemOutflow pathwayEndocrine communicationEndocrine systemEndocrine peptidesRegulatory peptides
2004
The bovine iris–ciliary epithelium expresses components of rod phototransduction
Ghosh S, Salvador-Silva M, Coca-Prados M. The bovine iris–ciliary epithelium expresses components of rod phototransduction. Neuroscience Letters 2004, 370: 7-12. PMID: 15489008, DOI: 10.1016/j.neulet.2004.07.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornArrestinBlotting, NorthernBlotting, WesternCattleCiliary BodyEpitheliumEye ProteinsGene ExpressionG-Protein-Coupled Receptor Kinase 1IrisLight Signal TransductionNeuronsPromoter Regions, GeneticProtein KinasesRetinal Rod Photoreceptor CellsReverse Transcriptase Polymerase Chain ReactionRhodopsinRNA, MessengerTransfectionConceptsCiliary epitheliumIris cellsBasal activityCommon embryonic originOcular ciliary epitheliumNeural retinaWestern blotRT-PCR amplificationRetinaEpitheliumSignificant stimulationBovine irisEmbryonic originStimulationBlotRod phototransductionTransient transfectionNorthern blotRhodopsin kinasePromoter activityDistal promoter elementLow levelsIrisReporter constructsLower vertebrates
2002
Bioinformatics and reanalysis of subtracted expressed sequence tags from the human ciliary body: Identification of novel biological functions.
Escribano J, Coca-Prados M. Bioinformatics and reanalysis of subtracted expressed sequence tags from the human ciliary body: Identification of novel biological functions. Molecular Vision 2002, 8: 315-32. PMID: 12355061.Peer-Reviewed Original ResearchMeSH KeywordsCiliary BodyComputational BiologyExpressed Sequence TagsEye ProteinsGene ExpressionHumansSequence Analysis, DNAConceptsSequence tagsUnidentified sequencesBLAST serviceDNA sequence comparisonsCell cycle regulationNon-coding regionsNon-redundant databaseBLAST search programNovel biological functionNovel candidate genesNovel physiological functionHuman Genome ProjectIndependent ESTsCircadian rhythm controlSubtractive hybridizationCellular signalingCycle regulationSequence comparisonAdditional genesDNA sequencesCytoskeleton structurePDB sequencesGenome ProjectCandidate genesBiological functions
2000
Expression of the TIGR gene in the iris, ciliary body, and trabecular meshwork of the human eye.
Huang W, Jaroszewski J, Ortego J, Escribano J, Coca-Prados M. Expression of the TIGR gene in the iris, ciliary body, and trabecular meshwork of the human eye. Ophthalmic Genetics 2000, 21: 155-69. PMID: 11035548, DOI: 10.1076/1381-6810(200009)21:3;1-z;ft155.Peer-Reviewed Original ResearchConceptsTIGR proteinTranscription/translation systemCanine pancreatic microsomal membranesPancreatic microsomal membranesSitu hybridization experimentsTissue-specific mannerTIGR genePattern of expressionCarboxy-terminus regionMajor protein bandsHybridization experimentsTerminus regionFusion proteinGlycosylation activityMolecular massProteinPNGase FDeglycosylation treatmentProtein bandsMicrosomal membranesTranslation systemO-glycosidaseJuvenile-onset primary open-angle glaucomaGenes
1999
Differential gene expression in the human ciliary epithelium
Coca-Prados M, Escribano J, Ortego J. Differential gene expression in the human ciliary epithelium. Progress In Retinal And Eye Research 1999, 18: 403-429. PMID: 10192520, DOI: 10.1016/s1350-9462(98)00026-3.Peer-Reviewed Original ResearchMeSH KeywordsCiliary BodyDNA, ComplementaryEpitheliumGene ExpressionGene LibraryHumansNeurosecretory SystemsConceptsDifferential gene expressionCell-specific expressionDiversity of functionsExpression of genesOcular ciliary bodySubtractive libraryNeuropeptide processing enzymesMolecular basisGene expressionCultured ciliary epithelial cellsCell differentiationCiliary epitheliumHuman ciliary epitheliumAntioxidative enzymesGenesCiliary epithelial cellsRegulatory peptidesLight-dark cycleEpithelial cellsMuscle cellsCiliary muscle cellsAutocrine mechanismExpressionPlasma proteinsParacrine mechanisms
1997
Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humorAssociation of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells
Coca-Prados M, Sánchez-Torres J, Peterson-Yantorno K, Civan M. Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells. The Journal Of Membrane Biology 1996, 150: 197-208. PMID: 8661780, DOI: 10.1007/s002329900044.Peer-Reviewed Original Research
1993
Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1
Martín-Alonso J, Ghosh S, Coca-Prados M. Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1. The Journal Of Biochemistry 1993, 114: 284-291. PMID: 8262911, DOI: 10.1093/oxfordjournals.jbchem.a124168.Peer-Reviewed Original ResearchConceptsOcular ciliary epitheliumGlutathione peroxidase cDNAMammalian eyeCorresponding amino acid sequencesDifferential gene expressionOpen reading frameAmino acid sequenceBovine glutathione peroxidasePeroxidase cDNAMolecular cloningOverall identityReading frameHuman GPxAcid sequenceGene expressionCellular formCiliary epitheliumFunctional roleAmino acidsTranscriptsOxidative damageGlutathione peroxidaseCloningOxidative stressExpression
1992
Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium
Hernando N, Martin-Alonso J, Ghosh S, Coca-Prados M. Isolation of a cDNA encoding a glutathione S-transferase (GST) class-π from the bovine ocular ciliary epithelium. Experimental Eye Research 1992, 55: 711-718. PMID: 1478280, DOI: 10.1016/0014-4835(92)90175-r.Peer-Reviewed Original ResearchMolecular Cloning of the Bovine CD9 Antigen from Ocular Ciliary Epithelial Cells1
Martín-Alonso J, Hernando N, Ghosh S, Coca-Prados M. Molecular Cloning of the Bovine CD9 Antigen from Ocular Ciliary Epithelial Cells1. The Journal Of Biochemistry 1992, 112: 63-67. PMID: 1339429, DOI: 10.1093/oxfordjournals.jbchem.a123866.Peer-Reviewed Original ResearchExpression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium
Coca-Prados M, Ghosh S, Gilula N, Kumar N. Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium. Current Eye Research 1992, 11: 113-122. PMID: 1374005, DOI: 10.3109/02713689209000061.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsGene productsCiliary epithelial cellsApical plasma membrane domainPlasma membrane domainsEpithelial cellsAlpha 3Plasma membrane regionGap junction genesPE cellsNorthern blot analysisGap junction proteinImmunofluorescence localization studiesAlpha 1 transcriptsIndirect immunofluorescence localization studiesIntact tissueMembrane domainsAlpha 1 mRNABeta 1Predominant transcriptGap junction mRNAMembrane regionsJunction genesLocalization studiesCell types
1991
Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion
Ghosh S, Hernando N, Martín‐Alonso J, Martin‐Vasallo P, Coca‐Prados M. Expression of multiple Na+,K+‐ATPase genes reveals a gradient of isoforms along the nonpigmented ciliary epithelium: Functional implications in aqueous humor secretion. Journal Of Cellular Physiology 1991, 149: 184-194. PMID: 1660898, DOI: 10.1002/jcp.1041490203.Peer-Reviewed Original Research
1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform