1999
Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptors
1998
Molecular Identification and Coexpression of Galanin and GalR‐1 Galanin Receptor in the Human Ocular Ciliary Epithelium: Differential Modulation of Their Expression by the Activation of α2‐ and β2‐Adrenergic Receptors in Cultured Ciliary Epithelial Cells
Ortego J, Coca‐Prados M. Molecular Identification and Coexpression of Galanin and GalR‐1 Galanin Receptor in the Human Ocular Ciliary Epithelium: Differential Modulation of Their Expression by the Activation of α2‐ and β2‐Adrenergic Receptors in Cultured Ciliary Epithelial Cells. Journal Of Neurochemistry 1998, 71: 2260-2270. PMID: 9832123, DOI: 10.1046/j.1471-4159.1998.71062260.x.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic alpha-AgonistsAqueous HumorCell LineCiliary BodyClonidineColforsinCulture Media, Serum-FreeEpithelial CellsFluorescent Antibody TechniqueGalaninHumansNorepinephrineProtein PrecursorsReceptors, Adrenergic, alphaReceptors, Adrenergic, betaReceptors, GalaninReceptors, NeuropeptideRNA, MessengerTetradecanoylphorbol AcetateConceptsGalanin mRNA expression
1997
Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium
Ortego J, Coca‐Prados M. Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1997, 69: 1829-1839. PMID: 9349525, DOI: 10.1046/j.1471-4159.1997.69051829.x.Peer-Reviewed Original ResearchMeSH KeywordsAspartic Acid EndopeptidasesCell LineCiliary BodyColforsinDexamethasoneDNA PrimersEnzyme InductionEpithelial CellsFurinGene Expression RegulationHumansIsoproterenolModels, BiologicalNerve Growth FactorsNeurosecretory SystemsNeurotensinPolymerase Chain ReactionProprotein Convertase 2Proprotein ConvertasesReceptors, NeurotensinSubtilisinsTetradecanoylphorbol AcetateTimololTranscriptional ActivationUp-RegulationConceptsOcular ciliary epitheliumDifferential gene inductionImportant physiological functionsHuman ocular ciliary epitheliumHuman cell linesTransduction pathwaysGene inductionGene expressionCiliary epitheliumBeta2-adrenergic receptorMolecular characterizationPhysiological functionsCell extractsODM-2Human ciliary epitheliumNeuroepithelial cellsSame inducersPhorbol esterCell linesNeuroendocrine tissuesExpressionGrowth factorParacrine mechanismsPC2 expressionNerve growth factorGene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1990
Regulation of protein phosphorylation in ocular ciliary epithelial cells by A, C and Ca2+calmodulin-dependent protein kinases
Lopez-Briones L, Wax M, Coca-Prados M. Regulation of protein phosphorylation in ocular ciliary epithelial cells by A, C and Ca2+calmodulin-dependent protein kinases. Experimental Eye Research 1990, 51: 277-286. PMID: 2119313, DOI: 10.1016/0014-4835(90)90024-o.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesCalcimycinCells, CulturedCiliary BodyEpitheliumEye ProteinsHumansIsoproterenolPeptide MappingPhosphorylationProtein Kinase CProtein KinasesTetradecanoylphorbol AcetateConceptsProtein kinaseLate phosphorylationCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseOcular ciliary epithelial cellsProtein phosphorylation eventsPMA treatmentDependent protein kinaseTwo-dimensional polyacrylamide gel electrophoresisIdentification of intracellularProtein kinase ATryptic peptide analysisHuman ocular ciliary epitheliumEndogenous phosphoproteinsPhosphorylation eventsProtein substrates