A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway
Shi C, Szczesniak A, Mao L, Jollimore C, Coca‐Prados M, Hung O, Kelly ME. A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway. British Journal Of Pharmacology 2003, 139: 475-486. PMID: 12788807, PMCID: PMC1573867, DOI: 10.1038/sj.bjp.0705266.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TransformedChloride ChannelsCiliaDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsEpithelial CellsGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsHumansMAP Kinase Signaling SystemProtein Kinase CReceptor, Adenosine A3Receptor, Cannabinoid, CB1ConceptsCB1 receptorsCl- currentSR 141716Receptor agonistNPCE cellsA3 adenosineWhole-cell patch-clamp recordingsCB1 receptor inverse agonistBasal Cl(-) currentCB1 receptor agonistA3 receptor agonistPatch-clamp recordingsHuman CB1 receptorReceptor-transfected cellsReceptor inverse agonistHuman nonpigmented ciliary epithelial cellsSignaling pathwaysAdenosine receptor antagonistNonpigmented ciliary epithelial cellsCiliary epithelial cell linePI3K inhibitorsMitogen-activated protein kinase kinase inhibitorBeta-adrenergic receptor kinaseProtein kinase kinase inhibitorEpithelial cell line