2004
Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium
Fidzinski P, Salvador-Silva M, Choritz L, Geibel J, Coca-Prados M. Inhibition of NHE-1 Na+/H+ exchanger by natriuretic peptides in ocular nonpigmented ciliary epithelium. American Journal Of Physiology - Cell Physiology 2004, 287: c655-c663. PMID: 15140751, DOI: 10.1152/ajpcell.00552.2003.Peer-Reviewed Original ResearchConceptsC-type natriuretic peptideAtrial natriuretic peptideBrain natriuretic peptideNatriuretic peptideIntraocular pressureCiliary epitheliumNHE activityNatriuretic peptides atrial natriuretic peptideInhibitory effectType B receptorsNHE-1 activityNonpigmented ciliary epitheliumCell layerGap junction blockersDose-dependent mannerInhibitors of NHEOcular ciliary epitheliumRate of intracellularHypotensive effectJunction blockersTrabecular meshworkMammalian eyeExchanger activityB receptorNHE-1
1996
Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1993
Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1
Martín-Alonso J, Ghosh S, Coca-Prados M. Cloning of the Bovine Plasma Selenium-Dependent Glutathione Peroxidase (GP) cDNA from the Ocular Ciliary Epithelium: Expression of the Plasma and Cellular Forms within the Mammalian Eye1. The Journal Of Biochemistry 1993, 114: 284-291. PMID: 8262911, DOI: 10.1093/oxfordjournals.jbchem.a124168.Peer-Reviewed Original ResearchConceptsOcular ciliary epitheliumGlutathione peroxidase cDNAMammalian eyeCorresponding amino acid sequencesDifferential gene expressionOpen reading frameAmino acid sequenceBovine glutathione peroxidasePeroxidase cDNAMolecular cloningOverall identityReading frameHuman GPxAcid sequenceGene expressionCellular formCiliary epitheliumFunctional roleAmino acidsTranscriptsOxidative damageGlutathione peroxidaseCloningOxidative stressExpression
1987
Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye
Coca-Prados M, López-Briones L. Evidence that the α and α(+) isoforms of the catalytic subunit of (Na+,K+)-ATPase reside in distinct ciliary epithelial cells of the mammalian eye. Biochemical And Biophysical Research Communications 1987, 145: 460-466. PMID: 3036129, DOI: 10.1016/0006-291x(87)91343-x.Peer-Reviewed Original ResearchConceptsCatalytic subunitCiliary epithelial cellsEpithelial cellsNPE cellsCultured ciliary epithelial cellsBasolateral surfaceImmunoblot analysisCiliary processesIntact ciliary processesMammalian eyeATPasePE cellsSubunitsPolyclonal antibodiesCanine kidneyCellsAlpha formAlphaIndirect immunofluorescenceIsoformsProteinBovine eyes
1985
Regulation of protein phosphorylation of the intermediate-sized filament vimentin in the ciliary epithelium of the mammalian eye.
Coca-Prados M. Regulation of protein phosphorylation of the intermediate-sized filament vimentin in the ciliary epithelium of the mammalian eye. Journal Of Biological Chemistry 1985, 260: 10332-10338. PMID: 4019517, DOI: 10.1016/s0021-9258(17)39252-9.Peer-Reviewed Original ResearchConceptsBeta-adrenergic agonistsMammalian eyeAlpha-adrenergic agonist phenylephrineBeta-adrenergic antagonist timololCiliary epitheliumCultured ciliary epithelial cellsCyclic AMPCellular cyclic AMPEpithelial-derived cellsAgonist phenylephrineCiliary epithelial cellsProstaglandin E1Catecholamine hormonesMicroM forskolinSerum-free mediumCiliary processesMonoclonal antibodiesRabbit ciliary processesVimentinAdenylate cyclaseEpithelial cellsPhosphorylation of vimentinIndirect immunofluorescence microscopyIntermediate-sized filamentsAgonists
1983
Ciliary epithelia of the mammalian eye in cultured explants
Kondo K, Coca-Prados M, Sears M. Ciliary epithelia of the mammalian eye in cultured explants. Cell And Tissue Research 1983, 233: 629-638. PMID: 6627352, DOI: 10.1007/bf00212230.Peer-Reviewed Original ResearchConceptsPigment granulesCultured explantsDulbecco's Modified Eagle's MediumFocal junctionsColumnar cellsFetal bovine serumJunctional complexesCuboidal cellsMammalian eyeCiliary epitheliumChannel-like structuresEpithelial cellsPlastic culture dishesNonpigmented cellsExplantsCellsBovine serumUltrastructural characteristicsOutermost layerPlastic dishesModified Eagle MediumPigmented epitheliumBasement membraneEagle's mediumCulture dishes