2005
FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner
Berry FB, O'Neill MA, Coca-Prados M, Walter MA. FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner. Molecular And Cellular Biology 2005, 25: 1415-1424. PMID: 15684392, PMCID: PMC548007, DOI: 10.1128/mcb.25.4.1415-1424.2005.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationCell NucleusChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneContractile ProteinsDNA-Binding ProteinsEye AbnormalitiesFilaminsForkhead Transcription FactorsHeLa CellsHeterochromatinHumansMicrofilament ProteinsMutationProtein BindingProto-Oncogene ProteinsTranscription FactorsTumor Cells, CulturedConceptsFilamin AHuman nonpigmented ciliary epithelial cellsFunction mutationsNonpigmented ciliary epithelial cellsAxenfeld-Rieger syndromeAutosomal dominant disorderCiliary epithelial cellsMelanoma cellsSkeletal disordersElevated levelsDominant disorderEpithelial cellsImportant transcription factorSkeletal phenotypeProtein filamin AFOXC1Inhibitory activityDisorders
2004
Identification of Target Genes Regulated by FOXC1 Using Nickel Agarose–Based Chromatin Enrichment
Tamimi Y, Lines M, Coca-Prados M, Walter MA. Identification of Target Genes Regulated by FOXC1 Using Nickel Agarose–Based Chromatin Enrichment. Investigative Ophthalmology & Visual Science 2004, 45: 3904-3913. PMID: 15505035, DOI: 10.1167/iovs.04-0628.Peer-Reviewed Original ResearchConceptsChromatin enrichmentIsolation of chromatinChromatin immunoprecipitation assaysTight electrostatic interactionUncharacterized genesChromatin complexesFOXC1 proteinNickel agaroseImmunoprecipitation assaysPoor quality sequencesTarget genesAntibody availabilityCellular eventsCell cycleHistidine residuesGenesFOXC1Biochemical analysisClonesPCR amplificationChromatinIndependent assaysOcular developmentProteinEpithelium cells