Identification of Target Genes Regulated by FOXC1 Using Nickel Agarose–Based Chromatin Enrichment
Tamimi Y, Lines M, Coca-Prados M, Walter MA. Identification of Target Genes Regulated by FOXC1 Using Nickel Agarose–Based Chromatin Enrichment. Investigative Ophthalmology & Visual Science 2004, 45: 3904-3913. PMID: 15505035, DOI: 10.1167/iovs.04-0628.Peer-Reviewed Original ResearchConceptsChromatin enrichmentIsolation of chromatinChromatin immunoprecipitation assaysTight electrostatic interactionUncharacterized genesChromatin complexesFOXC1 proteinNickel agaroseImmunoprecipitation assaysPoor quality sequencesTarget genesAntibody availabilityCellular eventsCell cycleHistidine residuesGenesFOXC1Biochemical analysisClonesPCR amplificationChromatinIndependent assaysOcular developmentProteinEpithelium cells