2012
Syndecan 4 Regulates FGFR1 Signaling in Endothelial Cells by Directing Macropinocytosis
Elfenbein A, Lanahan A, Zhou TX, Yamasaki A, Tkachenko E, Matsuda M, Simons M. Syndecan 4 Regulates FGFR1 Signaling in Endothelial Cells by Directing Macropinocytosis. Science Signaling 2012, 5: ra36. PMID: 22569333, PMCID: PMC3827948, DOI: 10.1126/scisignal.2002495.Peer-Reviewed Original ResearchConceptsFGF receptor 1Mitogen-activated protein kinaseFibroblast growth factor-2MAPK signalingSyndecan-4Inhibition of Rab5Receptor tyrosine kinasesEndothelial cell migrationHeparan sulfate proteoglycanSignal transductionProtein kinaseFGF2 stimulationEndothelial cellsMAPK activationTyrosine kinaseGrowth factor 2Genetic knockoutCell migrationReceptor complexMacropinocytosisClasses of receptorsSignalingRab5Factor 2Sulfate proteoglycan
2006
Syndecan‐4 plays a key role in the phosphorylation of protein kinase B / Akt
Partovian C, Simons M. Syndecan‐4 plays a key role in the phosphorylation of protein kinase B / Akt. The FASEB Journal 2006, 20: a1080-a1080. DOI: 10.1096/fasebj.20.5.a1080-a.Peer-Reviewed Original ResearchPhosphoinositide-dependent kinase 1Syndecan-4Akt phosphorylationProtein kinase B/AktPhosphorylation of Thr308Hydrophobic motifActivation loopRaft fractionsEndothelial cellsKinase 1Akt activationPhosphorylationMammalian targetSyndecansThr308AktGrowth factorCentral roleRegulationCellsKey roleMajor roleRictorNumber of processesSer473
2001
Syndecan-4-mediated signalling
Simons M, Horowitz A. Syndecan-4-mediated signalling. Cellular Signalling 2001, 13: 855-862. PMID: 11728825, DOI: 10.1016/s0898-6568(01)00190-5.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsFibroblast Growth FactorsIsoenzymesMacromolecular SubstancesMembrane GlycoproteinsModels, BiologicalMolecular Sequence DataPhosphatidylinositol 4,5-DiphosphatePhosphorylationProtein Kinase CProtein Kinase C-alphaProtein Structure, TertiaryProteoglycansSignal TransductionSyndecan-4ConceptsSyndecan familySyndecan-4Carboxy-terminal PDZPDZ adaptor proteinsUnique signaling functionsProtein kinase C alphaCore proteinExtracellular matrix proteinsHeparan sulfate chainsSoluble growth factorsTyrosine kinase receptorsAdaptor proteinCytoplasmic tailSignaling functionsTransmembrane proteoglycansProteoglycan functionMultimolecular complexesC alphaMatrix proteinsKinase receptorsPostsynaptic density-95Zona occludensGlycosaminoglycan chainsSulfate chainsProtein
2000
Synectin, syndecan‐4 cytoplasmic domain binding PDZ protein, inhibits cell migration
Gao Y, Li M, Chen W, Simons M. Synectin, syndecan‐4 cytoplasmic domain binding PDZ protein, inhibits cell migration. Journal Of Cellular Physiology 2000, 184: 373-379. PMID: 10911369, DOI: 10.1002/1097-4652(200009)184:3<373::aid-jcp12>3.0.co;2-i.Peer-Reviewed Original ResearchConceptsSyndecan-4Cytoplasmic domainSyndecan-4 cytoplasmic domainTwo-hybrid libraryDomain-mediated interactionsNovel binding partnerInhibits cell migrationGene familyPDZ proteinsBinding partnerDependent regulationSynectinImportant regulatorCell adhesionCell migrationCell growthGrowth rateGIPCMigrationRegulatorDomainProteinTransportersOverexpressionNeuropilins
1999
Phosphatidylinositol-4,5-bisphosphate Mediates the Interaction of Syndecan-4 with Protein Kinase C †
Horowitz A, Murakami M, Gao Y, Simons M. Phosphatidylinositol-4,5-bisphosphate Mediates the Interaction of Syndecan-4 with Protein Kinase C †. Biochemistry 1999, 38: 15871-15877. PMID: 10625452, DOI: 10.1021/bi991363i.Peer-Reviewed Original ResearchConceptsCytoplasmic tailSyndecan-4Protein kinase CalphaProtein kinase CExtracellular matrix proteinsActivation of PKCalphaGrowth factorExtracellular signalsDelta isoenzymesTransmembrane proteoglycansKinase CMatrix proteinsPhosphatidylinositolPKCalphaActivation mechanismCooperative activationBisphosphateActivationProteoglycansRecent studiesTailPICalphaSignalingAffinityThe Role of Syndecan Cytoplasmic Domain in Basic Fibroblast Growth Factor-dependent Signal Transduction*
Volk R, Schwartz J, Li J, Rosenberg R, Simons M. The Role of Syndecan Cytoplasmic Domain in Basic Fibroblast Growth Factor-dependent Signal Transduction*. Journal Of Biological Chemistry 1999, 274: 24417-24424. PMID: 10446222, DOI: 10.1074/jbc.274.34.24417.Peer-Reviewed Original ResearchConceptsCytoplasmic domainBasic fibroblast growth factorSyndecan-4Ectoplasmic domainSignal transductionSyndecan-4 cytoplasmic domainVector-transduced cellsSyndecan cytoplasmic domainsGrowth factor-dependent signal transductionHeparan sulfate attachment sitesTransmembrane/cytoplasmic domainsGlycosylphosphatidylinositol (GPI) anchor sequenceImmortalized human cellsGlypican-1Full-length sequencesAddition of bFGFCytoplasmic tailAnchor sequenceChimeric proteinMigration of cellsFibroblast growth factorLow-serum mediumHuman cellsGrowth assaysVC cellsMyocyte-dependent Regulation of Endothelial Cell Syndecan-4 Expression ROLE OF TNF-α*
Zhang Y, Pasparakis M, Kollias G, Simons M. Myocyte-dependent Regulation of Endothelial Cell Syndecan-4 Expression ROLE OF TNF-α*. Journal Of Biological Chemistry 1999, 274: 14786-14790. PMID: 10329676, DOI: 10.1074/jbc.274.21.14786.Peer-Reviewed Original ResearchConceptsSyndecan-4 expressionECV cellsProtein kinase C alphaCardiac myocytesGene familySyndecan-4 mRNATNF-alphaPosttranscriptional mechanismsGene expressionPrimary cardiac myocytesUnique memberC alphaHuman endothelial cellsSyndecan-4Western analysisMouse cardiac myocytesSyndecan-4 levelsNormal myoblastsDependent mannerNF-kappaBExpressionH9c2 cellsHypoxic conditionsTumor necrosisEndothelial cells
1998
Phosphorylation of the Cytoplasmic Tail of Syndecan-4 Regulates Activation of Protein Kinase Cα*
Horowitz A, Simons M. Phosphorylation of the Cytoplasmic Tail of Syndecan-4 Regulates Activation of Protein Kinase Cα*. Journal Of Biological Chemistry 1998, 273: 25548-25551. PMID: 9748216, DOI: 10.1074/jbc.273.40.25548.Peer-Reviewed Original ResearchRegulation of Syndecan-4 Phosphorylation in Vivo *
Horowitz A, Simons M. Regulation of Syndecan-4 Phosphorylation in Vivo *. Journal Of Biological Chemistry 1998, 273: 10914-10918. PMID: 9556568, DOI: 10.1074/jbc.273.18.10914.Peer-Reviewed Original ResearchConceptsCytoplasmic tailNovel PKC isozymesSyndecan-4Basic fibroblast growth factorProtein serine/threonine phosphatase type 1Serine/threonine phosphatasePKC isozymesSingle serine residuePhosphatase type 1Phosphatase inhibitor calyculinProtein kinase CNIH 3T3 fibroblastsThreonine phosphataseVivo phosphorylationEffects of bFGFSerine residuesTransmembrane proteoglycansConventional PKCFibroblast growth factorKinase CPhosphorylationPKC inhibitorPotential involvementGrowth factorIsozymes
1997
Macrophage-dependent regulation of syndecan gene expression.
Li J, Brown L, Laham R, Volk R, Simons M. Macrophage-dependent regulation of syndecan gene expression. Circulation Research 1997, 81: 785-96. PMID: 9351452, DOI: 10.1161/01.res.81.5.785.Peer-Reviewed Original ResearchConceptsBlood-derived macrophagesSyndecan expressionSyndecan geneMyocardial infarctionGene expressionRegulation of expressionSyndecan-1Op/op miceGranulocyte-macrophage colony-stimulating factorMacrophage colony-stimulating factorRat infarct modelColony-stimulating factorHeparin-binding growth factorsSyndecan-4 mRNASyndecan-1 mRNABiological processesMacrophage influxCellular responsesMacrophage contentSyndecan-4Right ventricleTissue injuryOp miceExtracellular matrixPrompt increase