2018
Neurotransmitter signaling through heterotrimeric G proteins: insights from studies in C. elegans.
Koelle MR. Neurotransmitter signaling through heterotrimeric G proteins: insights from studies in C. elegans. WormBook 2018, 2018: 1-52. PMID: 26937633, PMCID: PMC5010795, DOI: 10.1895/wormbook.1.75.2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsHeterotrimeric GTP-Binding ProteinsNeurotransmitter AgentsSignal TransductionConceptsHeterotrimeric G proteinsC. elegansG proteinsRic-8 proteinsNew molecular componentsG protein signalingG alpha proteinsNeurotransmitter releaseGenetic screenMammalian orthologsSmall molecule neurotransmittersIndividual receptor typesProtein signalingReceptor homologG alphaElegansDistant cellsAlpha proteinAdditional GPCRsGenetic studiesIndividual neural circuitsGαMolecular componentsGαqGαo
2006
Heterotrimeric G Protein Signaling: Getting inside the Cell
Koelle MR. Heterotrimeric G Protein Signaling: Getting inside the Cell. Cell 2006, 126: 25-27. PMID: 16839871, DOI: 10.1016/j.cell.2006.06.026.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneEndosomesHeterotrimeric GTP-Binding ProteinsModels, BiologicalPhosphatidylinositol 3-KinasesProtein BindingReproduction, AsexualSaccharomyces cerevisiaeSex AttractantsSignal Transduction
2004
RGS-7 Completes a Receptor-Independent Heterotrimeric G Protein Cycle to Asymmetrically Regulate Mitotic Spindle Positioning in C. elegans
Hess HA, Röper JC, Grill SW, Koelle MR. RGS-7 Completes a Receptor-Independent Heterotrimeric G Protein Cycle to Asymmetrically Regulate Mitotic Spindle Positioning in C. elegans. Cell 2004, 119: 209-218. PMID: 15479638, DOI: 10.1016/j.cell.2004.09.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell DivisionCentrosomeEmbryo, NonmammalianGTP-Binding Protein alpha SubunitsGuanine Nucleotide Exchange FactorsGuanosine TriphosphateHeterotrimeric GTP-Binding ProteinsMutationNuclear ProteinsProtein BindingRecombinant Fusion ProteinsRGS ProteinsRNA InterferenceSpindle ApparatusConceptsG protein functionRIC-8G proteinsProtein functionC. elegans embryosAsymmetric cell divisionG protein effectorsHeterotrimeric G proteinsMitotic spindle positioningG protein signalingG-protein cycleSeven-transmembrane receptorsGPR-1/2RGS domainElegans embryosGTPase activatorProtein effectorsProtein cycleMicrotubule forcesSpindle positioningProtein signalingCell cortexCell divisionGTP hydrolysisMitotic spindle
2002
An N-terminal Region of Caenorhabditis elegans RGS Proteins EGL-10 and EAT-16 Directs Inhibition of Gαo VersusGαq Signaling*
Patikoglou GA, Koelle MR. An N-terminal Region of Caenorhabditis elegans RGS Proteins EGL-10 and EAT-16 Directs Inhibition of Gαo VersusGαq Signaling*. Journal Of Biological Chemistry 2002, 277: 47004-47013. PMID: 12354761, DOI: 10.1074/jbc.m208186200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnimals, Genetically ModifiedBlotting, WesternCaenorhabditis elegansCaenorhabditis elegans ProteinsCell MembraneChromosomesEpitopesGTP-Binding Protein RegulatorsHelminth ProteinsHeterotrimeric GTP-Binding ProteinsImmunoblottingModels, BiologicalMolecular Sequence DataMutationPlasmidsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProteinsRGS ProteinsSequence Homology, Amino AcidSignal TransductionTime FactorsTransgenesConceptsN-terminal regionEGL-10EGL-30GOA-1EAT-16G protein signaling (RGS) proteinsN-terminalGPB-2RGS domainRGS proteinsC. elegansGbeta subunitsMembrane localizationSignaling proteinsN-terminal fragmentC-terminal fragmentGTPase activityTarget specificityBiochemical analysisProteinTarget selectivityFragment complexChimerasFragmentsDirect inhibition
2001
Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function
Chase D, Patikoglou G, Koelle M. Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function. Current Biology 2001, 11: 222-231. PMID: 11250150, DOI: 10.1016/s0960-9822(01)00071-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsGene ExpressionGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding Protein beta SubunitsGTP-Binding Protein RegulatorsGTP-Binding ProteinsHelminth ProteinsHeterotrimeric GTP-Binding ProteinsMiceNeuronsOvipositionRGS ProteinsSignal TransductionTransgenesConceptsRGS proteinsEGL-10EAT-16C. elegansG protein signaling (RGS) proteinsG protein heterotrimersC. elegans neuronsG protein signalingGPB-2Gbeta proteinsGTPase activatorNull mutantsRGS activityDouble mutantSignaling proteinsProtein signalingG proteinsPhysiological roleMutantsProteinKnockout animalsElegansObvious defectsRGSSubunits