2023
Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles
Olson A, Butt A, Christie N, Shelar A, Koelle M. Multiple Subthreshold GPCR Signals Combined by the G-Proteins Gαq and Gαs Activate the Caenorhabditis elegans Egg-Laying Muscles. Journal Of Neuroscience 2023, 43: 3789-3806. PMID: 37055179, PMCID: PMC10219013, DOI: 10.1523/jneurosci.2301-22.2023.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsMultiple G protein-coupled receptorsMuscle cellsMuscle activitySerotonin G protein-coupled receptorsDesigner G protein-coupled receptorsIntact animalsG proteinsEndogenous G protein-coupled receptorsIndividual G protein-coupled receptorsGPCR signalsCalcium activityEgg-laying musclesSerotoninIndividual neuronsDouble knockoutNeuronsBehavioral outcomesMuscleMost cellsG protein GαqCellsSubthreshold signalEgg-laying systemSER-1
2022
Using NeuroPAL Multicolor Fluorescence Labeling to Identify Neurons in C. elegans
Santiago E, Shelar A, Christie N, Lewis‐Hayre M, Koelle M. Using NeuroPAL Multicolor Fluorescence Labeling to Identify Neurons in C. elegans. Current Protocols 2022, 2: e610. PMID: 36521003, PMCID: PMC10257892, DOI: 10.1002/cpz1.610.Peer-Reviewed Original Research
2016
Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition
Collins KM, Bode A, Fernandez RW, Tanis JE, Brewer JC, Creamer MS, Koelle MR. Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition. ELife 2016, 5: e21126. PMID: 27849154, PMCID: PMC5142809, DOI: 10.7554/elife.21126.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsChloride ChannelsCholineFeedback, PhysiologicalFemaleGene Expression RegulationLocomotionMotor NeuronsMuscle ContractionOptogeneticsOvipositionPeriodicityReceptors, Biogenic AmineSerotoninSexual Behavior, AnimalSignal TransductionTyramineConceptsPassage of eggsUnderlying neural circuitsUv1 neuroendocrine cellsCommand neuronsMuscle contractionNeural circuitsNeuroendocrine cellsRhythmic activityBehavior circuitsCircuit activityCentral pattern generatorCircuit functionBody bendsFeedback inhibitionSlow locomotionPattern generatorNeuronsActivityVulva
2015
RNA ligation in neurons by RtcB inhibits axon regeneration
Kosmaczewski SG, Han SM, Han B, Meyer B, Baig HS, Athar W, Lin-Moore AT, Koelle MR, Hammarlund M. RNA ligation in neurons by RtcB inhibits axon regeneration. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 8451-8456. PMID: 26100902, PMCID: PMC4500288, DOI: 10.1073/pnas.1502948112.Peer-Reviewed Original Research
2013
LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans
Li P, Collins KM, Koelle MR, Shen K. LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans. ELife 2013, 2: e00378. PMID: 23539368, PMCID: PMC3601818, DOI: 10.7554/elife.00378.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCalcium SignalingCell Adhesion MoleculesFemaleGenotypeIntracellular Signaling Peptides and ProteinsMorphogenesisMuscle ContractionMusclesMutationNeurogenesisOvipositionParacrine CommunicationPhenotypeReceptors, NotchSignal TransductionSodium ChannelsSynapsesVulvaConceptsPrecise synaptic connectivityNon-target musclesForm synapsesMADD-2Types of musclePresynaptic neuronsSynaptic targetsSynaptic connectivityCardinal featuresNervous systemGuidance moleculesTarget cellsLIN-12/NotchUNC-40/DCCMuscleCell typesArm extensionCellsMuscle armsEctopic expressionDiverse cell typesDCCArmExpressionNeurons
2011
AGS-3 Alters Caenorhabditis elegans Behavior after Food Deprivation via RIC-8 Activation of the Neural G Protein Gαo
Hofler C, Koelle MR. AGS-3 Alters Caenorhabditis elegans Behavior after Food Deprivation via RIC-8 Activation of the Neural G Protein Gαo. Journal Of Neuroscience 2011, 31: 11553-11562. PMID: 21832186, PMCID: PMC3161416, DOI: 10.1523/jneurosci.2072-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCells, CulturedDrosophilaFood DeprivationGTP-Binding Protein alpha Subunits, Gi-GoGuanine Nucleotide Dissociation InhibitorsGuanine Nucleotide Exchange FactorsHumansNeuronsNuclear ProteinsProtein BindingSignal TransductionConceptsAGS-3GPR proteinsG proteinsGenetic epistasis experimentsG protein GαoRegulator domainGPR domainEpistasis experimentsBiochemical fractionationChemosensory neuronsBiological functionsBiological roleFood deprivationProteinCaenorhabditisDependent fashionFood-deprived animalsActivationVivoGαoGTPDomainAnimalsNeuronsDeprivation
2007
Biogenic amine neurotransmitters in C. elegans.
Chase DL, Koelle MR. Biogenic amine neurotransmitters in C. elegans. WormBook 2007, 1-15. PMID: 18050501, PMCID: PMC4781333, DOI: 10.1895/wormbook.1.132.1.Peer-Reviewed Original Research